Effect of ultrasound on herpes simplex virus infection in cell culture.
Effect of ultrasound on herpes simplex virus infection in cell culture.
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DOI：
10.1186/1743-422x-8-446
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发表时间：
2011-09-22
期刊：
影响因子：
4.8
通讯作者：
Yura Y
中科院分区：
文献类型：
--
作者：
Shintani M;Takahashi G;Hamada M;Okunaga S;Iwai S;Yura Y
超声波已被证明可以提高逆转录病毒、腺病毒和腺相关病毒的基因表达效率。研究了超声刺激细胞膜透化对溶瘤性单纯疱疹病毒1型（HSV-1）感染的影响。用HSV-1感染Vero猴肾细胞，并在吸附期后暴露于1 MHz超声。噬菌斑的数量显著大于未处理的对照。超声波和微泡的组合进一步增加了斑块数量。使用不同类型的HSV-1和口腔鳞状细胞癌（SCC）细胞获得了类似的结果。增加斑块数的超声强度、占空比和超声时间分别为0.5W/cm ~ 2、20%占空比和10 s。以2.0 W/cm 2的强度、50%占空比或持续40秒的具有微泡的超声降低了细胞活力。这些结果表明，超声促进溶瘤HSV-1进入细胞。这可能有助于提高溶瘤病毒治疗中HSV-1感染的效率。
Ultrasound has been shown to increase the efficiency of gene expression from retroviruses, adenoviruses and adeno-associated viruses. The effect of ultrasound to stimulate cell membrane permeabilization on infection with an oncolytic herpes simplex virus type 1 (HSV-1) was examined. Vero monkey kidney cells were infected with HSV-1 and exposed to 1 MHz ultrasound after an adsorption period. The number of plaques was significantly greater than that of the untreated control. A combination of ultrasound and microbubbles further increased the plaque number. Similar results were obtained using a different type of HSV-1 and oral squamous cell carcinoma (SCC) cells. The appropriate intensity, duty cycle and time of ultrasound to increase the plaque number were 0.5 W/cm2, 20% duty cycle and 10 sec, respectively. Ultrasound with microbubbles at an intensity of 2.0 W/cm2, at 50% duty cycle, or for 40 sec reduced cell viability. These results indicate that ultrasound promotes the entry of oncolytic HSV-1 into cells. It may be useful to enhance the efficiency of HSV-1 infection in oncolytic virotherapy.