Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification.
Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification.
复制标题
WLS，WNT9A，WNT5B和GPC4的不同要求在调节软骨细胞的成熟和内软骨骨化的时机方面。
DOI：
10.1016/j.ydbio.2016.11.016
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发表时间：
2017-01-15
影响因子：
2.7
通讯作者：
Liao EC
中科院分区：
文献类型：
--
作者：
Ling IT;Rochard L;Liao EC
下颌骨的形成需要软骨细胞在成骨前的渐进式形态学改变、增殖、分化和组织。Wnt信号通路参与调节骨发育和维持。注定要成为骨的软骨细胞需要Wnt适当地极化和定向来启动软骨内成骨程序。尽管典型的Wnt信号已经在骨发育的背景下得到了很好的研究，但非典型的Wnt信号在调节软骨成熟时间和随后的骨形成形成腹侧颅面结构中的作用尚未完全了解。在这里，我们研究了非规范Wnt信号通路（wls, gpc4， wnt5b和wnt9a）在调节斑马鱼梅克尔软骨成熟到成骨分化的作用。我们发现，壁的破坏导致颅面骨的严重损失，而缺乏gpc4， wnt5b和wnt9a导致严重延迟软骨内成骨。本研究证明了非规范Wnt通路在调节协调的腹侧软骨形态发生和骨化中的重要性。
Formation of the mandible requires progressive morphologic change, proliferation, differentiation and organization of chondrocytes preceding osteogenesis. The Wnt signaling pathway is involved in regulating bone development and maintenance. Chondrocytes that are fated to become bone require Wnt to polarize and orientate appropriately to initiate the endochondral ossification program. Although the canonical Wnt signaling has been well studied in the context of bone development, the effects of non-canonical Wnt signaling in regulating the timing of cartilage maturation and subsequent bone formation in shaping ventral craniofacial structure is not fully understood.. Here we examined the role of the non-canonical Wnt signaling pathway (wls, gpc4, wnt5b and wnt9a) in regulating zebrafish Meckel’s cartilage maturation to the onset of osteogenic differentiation. We found that disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification. This study demonstrates the importance of the non-canonical Wnt pathway in regulating coordinated ventral cartilage morphogenesis and ossification.