Investigation of the impact of PM2.5 on the ciliary motion of human nasal epithelial cells
Investigation of the impact of PM2.5 on the ciliary motion of human nasal epithelial cells
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PM2.5对人鼻上皮细胞纤毛运动影响的研究
DOI：
10.1016/j.chemosphere.2019.05.274
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发表时间：
2019-10-01
期刊：
影响因子：
8.8
通讯作者：
Chen, Tian
中科院分区：
文献类型：
--
作者：
Jia, Jiaxin;Xia, Jiao;Chen, Tian
鼻上皮为潜在的有害刺激提供了物理屏障。纤毛位于人鼻上皮细胞（HNEpCs）的顶端，在清除吸入的有害物质方面起着关键作用。睫状体搏动频率（CBF）和睫状体搏动模式（CBP）是反映睫状体搏动功能的两个重要指标。然而，细颗粒物（PM2.5）对CBF和CBP的影响仍然未知。我们的目的是评估PM2.5对HNEpCs纤毛搏动功能的影响及其潜在机制。PM2.5暴露12 h后，HNEpCs纤毛排列紊乱。不同浓度的PM2.5暴露后，纤毛覆盖率降低，基底细胞比例增加，并出现在HNEpCs的顶侧，这是无PM2.5暴露时几乎观察不到的。PM2.5暴露12 h后CBF增加，暴露24 h后相对低剂量组CBF增加，相对高剂量组CBF下降。CBF分为两种不同类型，在PM2.5暴露后有不同的变化。CBP表现出显著变化，其特征在于运动障碍指数增加。细胞ATP和线粒体膜电位的总水平下降后，12小时的PM2.5暴露，而没有变化的O-2消耗。总之，PM2.5影响HNEpCs的纤毛搏动功能，线粒体功能障碍可能在其中起重要作用。（C）2019 Elsevier Ltd.版权所有。
Nasal epithelium provides a physical barrier to potentially harmful stimuli. Cilia, which is on the apical side of the human nasal epithelial cells (HNEpCs), plays a critical role in removing inhaled harmful matter. Ciliary beat frequency (CBF) and ciliary beat pattern (CBP) are the two important indicators for ciliary beat function. However, impacts of the fine particulate matter (PM2.5) on CBF and CBP are still unknown. We aimed to evaluate the impact of PM2.5 on the ciliary beat function of the HNEpCs and its potential mechanisms. After exposed to PM2.5 for 12 h, cilia of HNEpCs were in disordered arrangement. The ciliary coverage rate was decreased after PM2.5 exposure of a series of concentration, while the proportion of basal cells was continuously increased and could be observed on the apical side of the HNEpCs which is hardly be observed without PM2.5 exposure. PM2.5 increased the CBF after 12 h exposure, while 24 h exposure increased the CBF at the relative lower dosage groups and then made a decrease at relative higher dosage groups. CBF were classified into two different types, which had different changes following PM2.5 exposure. CBP showed significant changes characterized as the increased dyskinesia index. Total levels of cellular ATP and the mitochondrial membrane potential were decreased following 12 h exposure of PM2.5, while no change was found in O-2 consumption. In conclusion, PM2.5 impact the ciliary beat function of HNEpCs, and the mitochondrial dysfunction might play an important role in it. (C) 2019 Elsevier Ltd. All rights reserved.