Cytokine Expression in the Spinal Cord Lesions in HTLV-l-associated Myelopathy
Cytokine Expression in the Spinal Cord Lesions in HTLV-l-associated Myelopathy
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HTLV-1 相关脊髓病脊髓病变中细胞因子的表达
DOI：
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发表时间：
1994
影响因子：
3.2
通讯作者：
M. Osame
中科院分区：
文献类型：
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作者：
F. Umehara;S. Izumo;Alicia T. Ronquillo;K. Matsumuro;E. Sato;M. Osame
用一组与白细胞介素-1/3（IL-1/3）、白细胞介素-6（IL-6）、肿瘤坏死因子（TNF）-α、干扰素（IFN）-α、IFN-β、IFN-γ和转化生长因子（TGF）-β反应的单克隆或多克隆抗体对5例HTLV-I相关性脊髓病/热带痉挛性截瘫尸检患者的脊髓进行免疫细胞化学染色。在病程较短的患者的脊髓中，IL-1/S、TNF-α和IFN-7在活动性-慢性炎性病变中的血管周围浸润性巨噬细胞、星形胶质细胞和小胶质细胞上表达。与此形成鲜明对比的是，我们很少注意到细胞因子的表达，除了IFN-7在非活动性慢性病变的患者持续时间较长。除了浸润的单核细胞外，这些细胞因子在小胶质细胞和星形胶质细胞上的原位表达表明胶质细胞参与炎症过程，特别是在活动性病变中。此外，细胞因子的表达随着病程的延长而沿着下调。
Immunocytochemical staining of spinal cords from five autopsied patients with HTLV-I-associated myelopathy/ tropical spastic paraparesis was performed using a panel of monoclonal or polyclonal antibodies reactive with interleukin-1/3 (IL-1/3), interleukin-6 (IL-6), tumor necrosis factor (TNF)-a, interferon (IFN)-a, IFN-/3, IFN--yand transforming growth factor (TGF)-/S. In the spinal cords of patients with a shorter duration of illness, IL-l/S, TNF-a, and IFN-7 were expressed on perivascular infiltrating macrophages, astrocytes and microglia in active-chronic inflammatory lesions. In striking contrast, we rarely noted cytokine expression except for IFN-7 in inactive-chronic lesions of patients with longer durations. In situ expression of these cytokines on microglia and astrocytes, in addition to infiltrating mononuclear cells, suggests that glial cells participate in the inflammatory process, especially in active lesions. In addition, the cytokine expression was gradually downregulated along with duration of illness.