Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals
Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals
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DOI：
10.1097/00004770-200203000-00009
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发表时间：
2002-03-01
影响因子：
4.2
通讯作者：
de Uzeda, M
中科院分区：
文献类型：
--
作者：
Siqueira, JF;Rôças, IN;de Uzeda, M
本研究的目的是比较两种器械技术和不同冲洗方法在体外产生的根管内细菌减少。分别采用交替旋转法（ARM）、手用镍钛锉和2.5%次氯酸钠（NaOCl）冲洗法、ARM联合2.5%次氯酸钠和柠檬酸冲洗法、ARM联合2.5%次氯酸钠和2%葡萄糖酸氯己定冲洗法、ARM联合2.5%葡萄和Greater Taper旋转锉，使用2.5%NaOCl作为冲洗液。通过使用ARM技术对对照组进行器械固定，并用无菌生理盐水冲洗。在制备前后对根管进行取样。连续稀释后，将样品接种到Mitis-Salivarius琼脂上，并对生长的殖民地形成单位进行计数。所有测试技术和溶液均显著减少根管内细菌细胞的数量（p < 0.05）。各实验组间无显著性差异（p < 0.05）。治疗组疗效优于对照组（P < 0.05）。这些发现支持了在化学机械制备过程中使用抗菌冲洗剂的重要性，无论使用何种溶液或仪器技术。
The purpose of this study was to compare the in vitro intracanal bacterial reduction produced by using two instrumentation techniques and different irrigation methods. Root canals inoculated with Enterococcus faecalis were prepared by using the following techniques and irrigants: alternated rotary motions (ARM) technique, hand nickel-titanium files and 2.5% sodium hypochlorite (NaOCl) as irrigant; ARM technique and combined irrigation with 2.5% NaOCl and citric acid; ARM technique and combined irrigation with 2.5% NaOCl and 2% chlorhexidine gluconate; and Greater Taper rotary files, using 2.5% NaOCl as irrigant. Controls were instrumented by using the ARM technique and irrigated with sterile saline. Canals were sampled before and after preparation. After serial dilution, samples were plated onto Mitis-Salivarius agar, and the colony forming units that were grown were counted. All test techniques and solutions significantly reduced the number of bacterial cells within the root canal (p < 0.05). There was no significant difference between the experimental groups (p < 0.05). Nonetheless, all of them were significantly more effective than the control group (p < 0.05). These findings support the importance of using antimicrobial irrigants during the chemomechanical preparation, regardless of the solutions or instrumentation techniques used.