Controlled microchannelling in dense collagen scaffolds by soluble phosphate glass fibers
Controlled microchannelling in dense collagen scaffolds by soluble phosphate glass fibers
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DOI：
10.1021/bm060715f
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发表时间：
2007-02-01
影响因子：
6.2
通讯作者：
Brown, Robert A.
中科院分区：
文献类型：
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作者：
Nazhat, Showan N.;Abou Neel, Ensanya A.;Brown, Robert A.
组织工程支架的一个问题是由于氧气和营养转移的限制而维持接种细胞的活力和功能。在整个支架中保持合适的氧浓度的方法将是可控地将微通道引入这些支架内。本研究探讨了将单向排列的可溶性磷酸盐基玻璃纤维（PGF）掺入致密胶原支架中。PGF是可降解的，并且它们的降解可以通过它们的化学性质和尺寸来控制。塑料压缩用于生产三种不同重量百分比的复合支架，同时保持大于80%的驻留细胞活力。通过热重分析以及进行形态学和机械特性的定量的PGF-胶原支架组合物。通过离子色谱法测量PGF降解，并通过超声成像和SEM验证通道形成。包被的微泡剂的自由移动证实了通道在性质上是连续的并且直径为30-40 μ m。致密天然胶原基质中的这些微通道可以在缺氧/灌注限制以及营养物质的运输和通过致密植入物形成血管中发挥重要作用。
A problem with tissue engineering scaffolds is maintaining seeded cell viability and function due to limitations of oxygen and nutrient transfer. An approach to maintain suitable oxygen concentrations throughout the scaffold would be to controllably incorporate microchannelling within these scaffolds. This study investigated the incorporation of unidirectionally aligned soluble phosphate based glass fibers (PGF) into dense collagen scaffolds. PGF are degradable, and their degradation can be controlled through their chemistry and dimensions. Plastic compression was used to produce composite scaffolds at three different weight percentage while maintaining greater than 80% resident cell viability. PGF-collagen scaffold composition was quantified through thermogravimetric analysis as well as being morphologically and mechanically characterized. PGF degradation was measured through ion chromatography, and channel formation was verified with ultrasound imaging and SEM. The free movement of coated microbubble agents confirmed the channels to be continuous in nature and of 30-40 mu m diameter. These microchannels in dense native collagen matrices could play an important role in hypoxia/perfusion limitations and also in the transportation of nutrients and potentially forming blood vessels through dense implants.