Sleep deprivation effects on growth factor expression in neonatal rats: A potential role for BDNF in the mediation of delta power
Sleep deprivation effects on growth factor expression in neonatal rats: A potential role for BDNF in the mediation of delta power
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DOI：
10.1152/jn.00894.2003
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发表时间：
2004-04-01
影响因子：
2.5
通讯作者：
O'Hara, BF
中科院分区：
文献类型：
--
作者：
Hairston, IS;Peyron, C;O'Hara, BF
睡眠的大脑在电生理和分子特性上不同于清醒的大脑，包括生长因子和立即早期基因（IEG）的表达。新生儿的睡眠结构和睡眠的稳态调节与成人不同。因此，本研究探讨了新生儿睡眠剥夺的独特稳态反应是否反映在IEG cFos，脑源性神经生长因子（BDNF）和碱性成纤维细胞生长因子（FGF 2）在皮质中的mRNA表达中。由于睡眠剥夺对发育中的大鼠有压力，我们还研究了皮质酮水平的增加是否会影响海马中生长因子的表达，已知海马对糖皮质激素水平敏感。在出生后第16、20和24天，对大鼠进行睡眠剥夺、无睡眠剥夺的母体分离、睡眠剥夺伴2小时恢复睡眠或无干预。在皮质和海马中定量mRNA表达。cFos在睡眠剥夺后增加，并且在2小时恢复睡眠后与对照水平相似，与年龄或脑区域无关。BDNF在P20和P24的皮质中被睡眠剥夺增加，而在海马中仅在P24增加。在所有年龄段的恢复睡眠期间，两个大脑区域的FGF2增加。我们的结论是，皮质BDNF的表达反映了成年人睡眠稳态反应的发病，而这两种生长因子的表达曲线表明轻度睡眠剥夺的营养效应。
The sleeping brain differs from the waking brain in its electrophysiological and molecular properties, including the expression of growth factors and immediate early genes (IEG). Sleep architecture and homeostatic regulation of sleep in neonates is distinct from that of adults. Hence, the present study addressed the question whether the unique homeostatic response to sleep deprivation in neonates is reflected in mRNA expression of the IEG cFos, brain-derived nerve growth factor ( BDNF), and basic fibroblast growth factor (FGF2) in the cortex. As sleep deprivation is stressful to developing rats, we also investigated whether the increased levels of corticosterone would affect the expression of growth factors in the hippocampus, known to be sensitive to glucocorticoid levels. At postnatal days 16, 20, and 24, rats were subjected to sleep deprivation, maternal separation without sleep deprivation, sleep deprivation with 2 h recovery sleep, or no intervention. mRNA expression was quantified in the cortex and hippocampus. cFos was increased after sleep deprivation and was similar to control level after 2 h recovery sleep irrespective of age or brain region. BDNF was increased by sleep deprivation in the cortex at P20 and P24 and only at P24 in the hippocampus. FGF2 increased during recovery sleep at all ages in both brain regions. We conclude that cortical BDNF expression reflects the onset of adult sleep-homeostatic response, whereas the profile of expression of both growth factors suggests a trophic effect of mild sleep deprivation.