DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells
DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells
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DOI：
10.1093/nar/gkh591
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发表时间：
2004-05-01
影响因子：
14.9
通讯作者：
Sakaguchi, K
中科院分区：
文献类型：
--
作者：
Kimura, S;Tahira, Y;Sakaguchi, K
以水稻为材料，采用北方杂交和原位杂交技术研究了CPD光裂合酶、UV-DDB 1、CSB、PCNA、RPA 32和FEN-1等基因的表达模式。CV. Nipponbare）。我们发现所有的基因都在富含增殖细胞的组织中表达，而只有CPD光裂合酶在非增殖组织中表达，如成熟叶片和根的伸长区。在光照条件下，观察到紫外线照射后，成熟叶片和根尖分生组织（RAM）中的DNA损伤、环丁烷嘧啶二聚体和（6-4）光产物的去除。在黑暗条件下，成熟叶片中的DNA损伤不能有效修复，而RAM中的DNA损伤能迅速消除。使用水稻22 K定制寡核苷酸DNA微阵列，我们比较了全球基因表达模式在茎尖分生组织（SAM）和成熟叶片。大多数切除修复基因在SAM中表达更强。这些结果表明，在非增殖细胞中，光复活是主要的DNA修复途径，而在增殖细胞中，光复活和切除修复都是活跃的。
We investigated expression patterns of DNA repair genes such as the CPD photolyase, UV-DDB1, CSB, PCNA, RPA32 and FEN-1 genes by northern hybridization analysis and in situ hybridization using a higher plant, rice (Oryza sativa L. cv. Nipponbare). We found that all the genes tested were expressed in tissues rich in proliferating cells, but only CPD photolyase was expressed in non-proliferating tissue such as the mature leaves and elongation zone of root. The removal of DNA damage, cyclobutane pyrimidine dimers and (6-4) photoproducts, in both mature leaves and the root apical meristem (RAM) was observed after UV irradiation under light. In the dark, DNA damage in mature leaves was not repaired efficiently, but that in the RAM was removed rapidly. Using a rice 22K custom oligo DNA microarray, we compared global gene expression patterns in the shoot apical meristem (SAM) and mature leaves. Most of the excision repair genes were more strongly expressed in SAM. These results suggested that photoreactivation is the major DNA repair pathway for the major UV-induced damage in non-proliferating cells, while both photoreactivation and excision repair are active in proliferating cells.