Rational collaborative ablation of bacterial biofilms ignited by physical cavitation and concurrent deep antibiotic release
Rational collaborative ablation of bacterial biofilms ignited by physical cavitation and concurrent deep antibiotic release
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DOI：
10.1016/j.biomaterials.2020.120341
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发表时间：
2020-12-01
期刊：
影响因子：
14
通讯作者：
Hu, Xianglong
中科院分区：
文献类型：
--
作者：
Cao, Bing;Lyu, Xiaoming;Hu, Xianglong
细菌生物膜具有细胞外聚合物质，可以保护细菌免受外部威胁，这是人类健康的一个顽固问题。在此，制造了一种可气化的纳米液滴来消融金黄色葡萄球菌（S. aureus）生物膜。在近红外脉冲激光照射下，纳米液滴可以气化，产生破坏性气体冲击波，进一步增强初始声空化效应，从而协同破坏保护性生物膜并杀死常驻细菌。更重要的是，气化可以进一步促进深层生物膜中抗生素的释放，从而消灭残留细菌。纳米粒不仅具有深层生物膜穿透能力和高效消融生物膜的能力，而且具有良好的生物相容性，且没有可检测到的副作用。体内小鼠植入模型表明纳米液滴可以在金黄色葡萄球菌感染的植入位点积聚。在脉冲激光治疗后，纳米液滴通过气体冲击波增强空化和深层抗生素释放的协同作用，有效地消除植入导管中的细菌生物膜。当前具有协同物理和化学治疗方式的相变纳米粒有望对抗具有耐药性的复杂细菌生物膜，这为生物医学中的生物膜抑制提供了替代视角。
Bacteria biofilm has extracellular polymeric substances to protect bacteria from external threats, which is a stubborn problem for human health. Herein, a kind of gasifiable nanodroplet is fabricated to ablate Staphylococcus aureus (S. aureus) biofilm. Upon NIR pulsed laser irradiation, the nanodroplets can gasify to generate destructive gas shockwave, which further potentiates initial acoustic cavitation effect, thus synergistically disrupting the protective biofilm and killing resident bacteria. More importantly, the gasification can further promote antibiotic release in deep biofilm for residual bacteria eradication. The nanodmplets not only exhibit deep biofilm penetration capacity and high potency to ablate biofilms, but also good biocompatibility without detectable side effects. In vivo mouse implant model indicates that the nanodroplets can accumulate at the S. aureus infected implant sites. Upon pulsed laser treatment, the nanodroplets efficiently eradicate bacteria biofilm in implanted catheter by synergistic contribution of gas shockwave-enhanced cavitation and deep antibiotic release. Current phase changeable nanodmplets with synergistic physical and chemical therapeutic modalities are promising to combat complex bacterial biofilms with drug resistance, which provides an alternative visual angle for biofilm inhibition in biomedicine.