In vivo evaluation of poly(N-isopropylacrylamide) (PNIPAM)-grafted gelatin as an in situ-formable scaffold
In vivo evaluation of poly(N-isopropylacrylamide) (PNIPAM)-grafted gelatin as an in situ-formable scaffold
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DOI：
10.1007/s10047-004-0265-9
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发表时间：
2005-02
影响因子：
1.3
通讯作者：
S. Ohya;Y. Nakayama;T. Matsuda
中科院分区：
文献类型：
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作者：
S. Ohya;Y. Nakayama;T. Matsuda
我们研究了通过准活性自由基接枝聚合制备的具有约34°C的较低临界溶液温度的聚（N-异丙基丙烯酰胺）接枝明胶（PNIPAM-明胶）是否可以用作原位可成形的三维细胞外基质或细胞支架。将在Dulbecco改良Eagle培养基溶液中用荧光染料和PNIPAM-明胶染色的成纤维细胞的混合物注射到Wistar大鼠的皮下组织中，并立即形成白色不透明的细胞掺入凝胶。成纤维细胞注射后立即呈球形，均匀分布在凝胶中。注射后2周，凝胶中的成纤维细胞扩散并增殖。注射后一天，在凝胶周围观察到许多巨噬细胞和嗜酸性粒细胞。随着植入期的进行，炎症反应消退。注射后一周，天然组织中的成纤维细胞和巨噬细胞迁移到凝胶中。注射后6 - 12周，在实体组织中间歇性观察到一定程度的钙化。植入后6周，凝胶的重量减少到最初注射样品重量的几乎一半。PNIPAM-明胶作为可注射支架的潜在用途进行了讨论。
We examined whether poly(N-isopropylacrylamide)-grafted gelatin (PNIPAM-gelatin) with a lower critical solution temperature of approximately 34°C, which was prepared by quasi-living radical graft polymerization, can serve as an in situ-formable three-dimensional extracellular matrix or cell scaffold. A mixture of fibroblasts stained with fluorescent dye and PNIPAM-gelatin in Dulbecco’s modified Eagle’s medium solution was injected into the subcutaneous tissue of Wistar rats, and immediately formed a white, opaque cell-incorporated gel. Fibroblasts immediately after injection were spherical in shape and were homogeneously distributed in the gel. Fibroblasts in the gel 2 weeks after injection had spread and proliferated. One day after injection, many macrophages and neutrophiles were observed around the gel. As the implantation period proceeded, the inflammation reaction subsided. One week after injection, fibroblasts in the native tissue and macrophages migrated into the gel. From 6 to 12 weeks after injection, some degree of calcification in the solid tissue was intermittently observed. The weight of the gel 6 weeks after implantation was reduced to almost one-half of the weight of the originally injected sample. The potential usefulness of PNIPAM-gelatin as an injectable scaffold is discussed.