Effect of cumulus cell removal of in vitro matured bovine oocytes prior to in vitro fertilization on subsequent cleavage rate
Effect of cumulus cell removal of in vitro matured bovine oocytes prior to in vitro fertilization on subsequent cleavage rate
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DOI：
10.1016/s0093-691x(01)00717-8
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发表时间：
2002-03-01
期刊：
影响因子：
2.8
通讯作者：
Bevers, MM
中科院分区：
文献类型：
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作者：
Fatehi, AN;Zeinstra, EC;Bevers, MM
本研究的目的是确定成熟牛卵母细胞体外受精前去除卵丘细胞对卵裂率的影响。剥去的成熟卵母细胞在有或没有松散的积云细胞、积云细胞条件的体外受精培养基（CCCM）、炭处理的CCCM和添加终浓度为150 ng/ml的黄体酮的炭处理的CCCM中受精。与精子孵育18小时后，将推定胚胎培养在BRL单层上，并于第4天评估卵裂胚胎的百分比。体外受精前去除卵丘细胞可显著降低卵裂率（剥落卵母细胞为25%，而卵丘-卵母细胞复合物（COCs）为56%）。松散积云细胞的加入部分恢复了剥落的效果（卵裂率：被剥落的卵母细胞补充松散积云细胞的卵裂率为37%，而被剥落的卵母细胞为27%，COCs为58%）。CCCM对体外受精前剥去的卵母细胞的卵裂率也有积极影响（在CCCM中受精的剥去的卵母细胞为36%，而剥去的卵母细胞为14%）。用木炭处理CCCM导致其对卵裂率的影响完全丧失（在木炭处理的CCCM中受精的脱毛卵母细胞为18%，而在CCCM中受精的脱毛卵母细胞为34%）。在炭处理的CCCM中添加黄体酮，部分恢复了炭处理导致的卵裂率的降低（在炭处理的CCCM中，脱毛卵母细胞受精率为27%，而在炭处理的CCCM中，脱毛卵母细胞受精率为14%，在CCCM中，脱毛卵母细胞受精率为36%）。综上所述，体外受精前去除积云细胞会减少这些细胞分泌的一种因子，从而对卵裂率产生不利影响。这个因素可能是黄体酮。(C) 2002爱思唯尔科学有限公司版权所有。
The aim of this study is to identify the effect of cumulus cells removal prior to the in vitro fertilization of matured bovine oocytes on cleavage rate. Denuded, matured oocytes were fertilized in presence or absence of loose cumulus cells, cumulus cell conditioned IVF medium (CCCM), charcoal-treated CCCM and charcoal-treated CCCM supplemented with progesterone at a final concentration of 150 ng/ml. After 18 h of incubation with sperm, the presumptive embryos were cultured on a BRL monolayer and the percentage of cleaved embryos was evaluated on Day 4.Removal of cumulus cells prior to IVF significantly reduced the cleavage rate (25% for denuded oocytes versus 56% for cumulus-oocyte complexes (COCs)). The addition of loose cumulus cells partially restored the effect of denudation (cleavage rate: 37% for denuded oocytes supplemented with loose cumulus cells versus 27% for denuded oocytes and 58% for COCs). CCCM also had a positive effect on the cleavage rate of oocytes denuded prior to IVF (36% for denuded oocytes fertilized in CCCM versus 14% for denuded oocytes). Treating the CCCM with charcoal resulted in complete loss of its effect on cleavage rate ( 18% for denuded oocytes fertilized in charcoal-treated CCCM versus 34% for denuded oocytes fertilized in CCCM). The addition of progesterone to charcoal-treated CCCM partially restored the reduction of the cleavage rate caused by charcoal treatment (27% for denuded oocytes fertilized in charcoal-treated CCCM supplemented with progesterone versus 14% for denuded oocytes fertilized in charcoal-treated CCCM and 36% for denuded oocytes fertilized in CCCM). In conclusion, removal of cumulus cells prior to IVF adversely affects the cleavage rate through loss of a factor secreted by these cells. This factor probably is progesterone. (C) 2002 Elsevier Science Inc. All rights reserved.