Chemical and Biological Dynamics Using Droplet-Based Microfluidics
Chemical and Biological Dynamics Using Droplet-Based Microfluidics
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DOI：
10.1146/annurev-anchem-061516-045219
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
deMello, Andrew J.
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文献类型：
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作者：
Dressler, Oliver J.;Casadevall i Solvas, Xavier;deMello, Andrew J.
近年来，基于液滴的微流控技术在各种化学和生物分析中得到了越来越多的使用。然而，从这些平台获取动态数据仍然具有挑战性，因为这往往需要在大范围的间隔(从毫秒到几个小时)内多次读取相同的液滴(可能有数千个)。在这篇综述中，我们介绍了微流控液滴形成和操纵的基本技术，以及这些领域的最新进展。然后，我们讨论了广泛的分析方法，这些方法已经成功地用于液滴中的分析物检测。最后，我们强调了各种研究，在这些研究中，基于液滴的微流控策略使原本无法探索的动态系统的表征成为可能。
Recent years have witnessed an increased use of droplet-based microfluidic techniques in a wide variety of chemical and biological assays. Nevertheless, obtaining dynamic data from these platforms has remained challenging, as this often requires reading the same droplets (possibly thousands of them) multiple times over a wide range of intervals (from milliseconds to hours). In this review, we introduce the elemental techniques for the formation and manipulation of microfluidic droplets, together with the most recent developments in these areas. We then discuss a wide range of analytical methods that have been successfully adapted for analyte detection in droplets. Finally, we highlight a diversity of studies where droplet-based microfluidic strategies have enabled the characterization of dynamic systems that would otherwise have remained unexplorable.