The mRNA coding for Xenopus glutamate receptor interacting protein 2 (XGRIP2) is maternally transcribed, transported through the late pathway and localized to the germ plasm
The mRNA coding for Xenopus glutamate receptor interacting protein 2 (XGRIP2) is maternally transcribed, transported through the late pathway and localized to the germ plasm
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DOI：
10.1016/j.bbrc.2007.02.059
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发表时间：
2007-04-20
影响因子：
3.1
通讯作者：
Komiya, Tohru
中科院分区：
文献类型：
--
作者：
Kaneshiro, Kazuki;Miyauchi, Maki;Komiya, Tohru
使用大规模原位杂交筛选，我们发现，非洲爪蟾谷氨酸受体相互作用蛋白2（XGRIP 2）的mRNA编码定位于非洲爪蟾的种质。mRNA在卵母细胞中进行母系转录，并在成熟过程中通过VegT和Vg 1 mRNA转运的晚期途径转运至植物种质。在mRNA的3 '-非翻译区（UTR）中，存在E2和VM 1定位基序簇，据报道这些基序存在于被归类为晚期途径组的mRNA中。与原位杂交的胚胎切片，信号可以检测到迁移的推定原始生殖细胞（pPGCs）的细胞质中，直到第35阶段。在第40阶段，当细胞停止迁移并到达背侧肠系膜时，信号消失。将讨论XGRIP 2在爪蟾pPGC中的可能作用。(c)2007年爱思唯尔公司All rights reserved.
Using a large-scale in situ hybridization screening, we found that the mRNA coding for Xenopus glutamate receptor interacting protein 2 (XGRIP2) was localized to the germ plasm of Xenopus laevis. The mRNA is maternally transcribed in oocytes and, during maturation, transported to the vegetal germ plasm through the late pathway where VegT and Vg1 mRNAs are transported. In the 3'-untranslated region (UTR) of the mRNA, there are clusters of E2 and VM1 localization motifs that were reported to exist in the mRNAs classified as the late pathway group. With in situ hybridization to the sections of embryos, the signal could be detected in the cytoplasm of migrating presumptive primordial germ cells (pPGCs) until stage 35. At stage 40, when the cells cease to migrate and reach the dorsal mesentery, the signal disappeared. A possible role of XGRIP2 in pPGCs of Xenopus will be discussed. (c) 2007 Elsevier Inc. All rights reserved.