The amyloid precursor-like protein (APLP) gene maps to the long arm of human chromosome 19.
The amyloid precursor-like protein (APLP) gene maps to the long arm of human chromosome 19.
复制标题
淀粉样前体样蛋白 (APLP) 基因定位于人类 19 号染色体的长臂。
DOI：
10.1006/geno.1993.1047
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Tanzi,RE
中科院分区：
文献类型：
--
作者：
Wasco,W;Brook,JD;Tanzi,RE
我们最近从小鼠大脑文库中分离出一种 cDNA，其编码的蛋白质的预测氨基酸序列与淀粉样蛋白 β 蛋白前体 (APP; 16) 的氨基酸序列有 42% 相同，64% 相似。这种由 653 个氨基酸组成的淀粉样前体样蛋白 (APLP) 在整体结构和氨基酸序列上与 APP 相似。蛋白质的三个不同区域的氨基酸同源性特别强，同一性分别为 47%、54% 和 56% (16)。所有这三个区域在果蝇 APP 样基因 APPL 中也是保守的 (11)。值得注意的是，12个半胱氨酸残基和一个N-糖基化位点在APLP和APP的胞外部分是保守的，并且网格蛋白结合结构域在胞质结构域中是保守的。据报道，大鼠睾丸中编码 APP 样基因的部分 cDNA 中的细胞质结构域也是保守的 (17)，这些数据表明 APLP 和 APP 是高度保守的基因家族的成员。用 EcoR1 消化来自 31 个已知核型的人类啮齿动物体细胞系的一组 DNA。然后用人 APLP cDNA 克隆探测这些 DNA，杂交模式与 APLP 基因座在 19 号染色体上的分配一致。17 参考文献，图 1。
We have recently isolated a cDNA from a mouse brain library that encodes a protein whose predicted amino acid sequence is 42% identical and 64% similar to that of the amyloid [beta] protein precursor (APP; 16). This 653-amino-acid amyloid precursor-like protein (APLP) is similar to APP in overall structure as well as amino acid sequence. The amino acid homologies are particularly strong in three distinct regions of the proteins where the identities are 47, 54, and 56% (16). All three of these regions are also conserved in the Drosophila APP-like gene, APPL (11). Notably, 12 cysteine residues and a N -glyco-sylation site are conserved in the extracellular portion of APLP and APP, and a clathrin-binding domain is conserved in the cytoplasmic domain. The cytoplasmic domain is also conserved in a partial CDNA reported to encode an APP-like gene in rat testes (17), These data suggest that APLP and APP are members of a highly conserved gene family. A panel of DNAs from 31 human-rodent somatic cell lines of known karyotype was digested with EcoR1. These DNAs were then probed with the human APLP cDNA clone and the hybridization pattern was consistent with the assignment of the APLP locus to chromosome 19. 17 refs., 1 fig.