Raman to the limit: tip-enhanced Raman spectroscopic investigations of a single tobacco mosaic virus
Raman to the limit: tip-enhanced Raman spectroscopic investigations of a single tobacco mosaic virus
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DOI：
10.1002/jrs.2123
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发表时间：
2009-03-01
影响因子：
2.5
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
--
作者：
Cialla, Dana;Deckert-Gaudig, Tanja;Popp, Juergen
发展病毒的快速识别技术是当前的一个重要研究课题。传统的病毒检测和鉴定一般基于各种不同的微生物方法。然而，这些技术不适用于单个病毒颗粒的分析。因此，我们的目标是建立尖端增强拉曼散射(TERS)，提供空间分辨率低于50 nm的振动光谱信息，在分子水平上表征单个病毒。在这里，据我们所知，我们第一次报告了烟草花叶病毒的层级谱，显示了这项技术的巨大能力。然而，用于快速和直接检测不同种类的单一病毒的TIERS技术的应用正在开发中，这对广泛的分析领域是有用的。版权所有(C)2008 John Wiley&Sons，Ltd.
The development of fast identification techniques of viruses is an ongoing important research topic. Conventional virus detection and identification is generally based on various different microbiological methods. However, these techniques are not suitable for the analysis of single virus particles. Therefore, our goal is to establish tip-enhanced Raman scattering (TERS), providing vibrational spectroscopic information with a spatial resolution less than 50 nm, to characterize single viruses at a molecular level. Here we report, to the best of our knowledge for the first time, about TIERS spectra of a tobacco mosaic virus, showing the great capability of this technique. However, the application of the TIERS technique for a rapid and direct detection of different species of single viruses is under development, which is useful for a wide range of analytical fields. Copyright (C) 2008 John Wiley & Sons, Ltd.