Sequence determination of the mumps virus HN gene.
Sequence determination of the mumps virus HN gene.
复制标题
腮腺炎病毒HN基因的序列测定。
DOI：
10.1016/0042-6822(88)90544-2
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Goodman,HM
中科院分区：
文献类型：
--
作者：
Waxham,MN;Aronowski,J;Server,AC;Wolinsky,JS;Smith,JA;Goodman,HM
用免疫亲和层析法纯化腮腺炎病毒血凝素-神经氨酸酶蛋白（HN），并用溴化氰和胰蛋白酶联合作用使HN蛋白片段化。通过HPLC分离所得肽，并通过自动Edman降解测序。使用此HN特异性氨基酸序列数据，产生简并寡核苷酸，随后用于筛选腮腺炎病毒cDNA文库以分离HN特异性克隆。测定了HN基因的全序列。单顺反子HN mRNA长约1900个核苷酸，编码582个氨基酸的单个开放阅读框。HN蛋白在其N-末端具有独特的19个氨基酸的疏水延伸，其显然将蛋白锚定在病毒包膜中。腮腺炎病毒HN蛋白质序列与其它已知副粘病毒HN蛋白质序列的比较表明腮腺炎病毒与SV-5最密切相关，其次是NDV、副流感病毒3和仙台病毒。
The hemagglutinin-neuraminidase protein (HN) of mumps virus was purified by immunoaffinity chromatography and fragmented by the combined action of CNBr and trypsin. The resulting peptides were separated by HPLC and sequenced by automated Edman degradation. Using this HN-specific amino acid sequence data, a degenerated oligo-nucleotide was produced and subsequently used to screen a mumps virus cDNA library to isolate HN-specific clones. The complete nucleotide sequence of the HN gene was determined. The monocistronic HN mRNA is approximately 1900 nucleotides long and encodes a single open reading frame of 582 amino acids. The HN protein has a unique hydrophobic stretch of 19 amino acids at its N-terminus that apparently anchors the protein in the viral envelope. A comparison of the mumps virus HN protein sequence with the sequences of the other known paramyxovirus HNs indicates that mumps virus is most closely related to SV-5, followed in decreasing order by NDV, parainfluenza virus 3, and Sendai virus.