Protocol to Generate Ureteric Bud Structures from Human iPS Cells
Protocol to Generate Ureteric Bud Structures from Human iPS Cells
复制标题
从人类 iPS 细胞生成输尿管芽结构的方案
DOI：
10.1007/978-1-4939-9021-4_10
复制
发表时间：
2019
影响因子：
--
通讯作者：
Osafune Kenji
中科院分区：
文献类型：
--
作者：
Mae Shin-Ichi;Ryosaka Makoto;Osafune Kenji
从人诱导多能干细胞（hiPSC）产生输尿管芽（UB），其是产生肾集合管和下尿路的肾祖细胞，为研究UB和肾脏疾病的发展提供了细胞来源。在这里，我们描述了一个逐步和有效的二维分化方法的hiPSCs到沃尔夫管（WD）细胞。我们还描述了如何产生三维WD上皮结构，可以分化成UB样结构。
The generation of ureteric bud (UB), which is the renal progenitor that gives rise to renal collecting ducts and lower urinary tract, from human-induced pluripotent stem cells (hiPSCs) provides a cell source for studying the development of UB and kidney disease. Here we describe a stepwise and efficient two-dimensional differentiation method of hiPSCs into Wolffian duct (WD) cells. We also describe how to generate three-dimensional WD epithelial structures that can differentiate into UB-like structures.