Modulation of O-Glycans and N-Glycans on Murine CD8 T Cells Fails to Alter Annexin V Ligand Induction by Galectin 11
Modulation of O-Glycans and N-Glycans on Murine CD8 T Cells Fails to Alter Annexin V Ligand Induction by Galectin 11
复制标题
调节鼠 CD8 T 细胞上的 O-聚糖和 N-聚糖未能改变半乳糖凝集素 11 对膜联蛋白 V 配体的诱导作用
作者：
D. A. Carlow;Michael J. Williams;H. Ziltener
感染后胸腺阴性选择和应答的T细胞寡克隆的收缩代表了维持T细胞稳态所需的重要细胞消融过程。有人提出，凝集素1主要通过与细胞表面携带核心2 （C2GnT1）支链o -聚糖的糖蛋白上的乳糖基序列相互作用来参与这些过程。根据该模型，由凝集素1交联的特异性T细胞表面蛋白可诱导未成熟胸腺细胞和活化T细胞的信号转导、配体再分配和凋亡。研究了支链o -聚糖或复合n -聚糖中含有的乳糖基残基对小鼠CD8 T细胞中凝集素1结合和膜联蛋白V配体诱导的影响。在以下条件下，凝集素结合和凝集素诱导的膜联蛋白V配体表达均未受到干扰：1)CD8 T细胞活化/培养时，IL-2和IL-4诱导的C2GnT1活性存在差异；2)活化的CD8+ T细胞缺乏C2GnT1表达；或3)复合n -聚糖的形成被苦马豆素阻断。在改变O-或复合n -聚糖上乳胺丰度的条件下，维持凝集素1结合并诱导膜联蛋白V表达表明，凝集素1介导的细胞凋亡既不是C2GnT1活性波动的简单功能，也不是CD8 T细胞活化后收缩的一般C2GnT1依赖机制。
Thymic negative selection and contraction of responding T cell oligoclones after infection represent important cell ablation processes required for maintaining T cell homeostasis. It has been proposed that galectin 1 contributes to these processes through interaction with lactosyl sequences principally on cell surface glycoproteins bearing core 2 (C2GnT1)-branched O-glycans. According to this model, specific T cell surface proteins cross-linked by galectin 1 induce signaling, ligand redistribution, and apoptosis in both immature thymocytes and activated T cells. The influence of lactosyl residues contained in branched O-glycans or complex N-glycans on galectin 1 binding and induction of annexin V ligand in murine CD8 T cells was assessed. Neither galectin binding nor galectin-induced expression of annexin V ligand was perturbed under conditions in which: 1) C2GnT1 activity was differentially induced by CD8 T cell activation/culture with IL-2 vs IL-4; 2) activated CD8+ T cells lacked C2GnT1 expression; or 3) complex N-glycan formation was blocked by swainsonine. The maintenance of galectin 1 binding and induced annexin V expression under conditions that alter lactosamine abundance on O- or complex N-glycans suggest that galectin 1-mediated apoptosis is neither a simple function of fluctuating C2GnT1 activity nor a general C2GnT1-dependent mechanism underlying contraction of CD8 T cells subsequent to activation.
DOI：
10.1016/s0021-9258(18)68157-8
发表时间：
1988-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Piller;V. Piller;R. Fox;Minoru Fukuda
通讯作者：
F. Piller;V. Piller;R. Fox;Minoru Fukuda
L14 S 型凝集素编码基因携带无效突变的小鼠的正常发育。
DOI：
10.1242/dev.119.4.1229
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Poirier,F;Robertson,EJ
通讯作者：
Robertson,EJ
影响因子：
32.4
作者：
Ellies, LG;Tsuboi, S;Marth, JD
通讯作者：
Marth, JD