Reduced quality of bovine embryos cultured in media conditioned by exposure to an inflamed endometrium
Reduced quality of bovine embryos cultured in media conditioned by exposure to an inflamed endometrium
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DOI：
10.1111/j.1751-0813.2008.00326.x
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发表时间：
2008-08-01
影响因子：
1.1
通讯作者：
Gilbert, R.
中科院分区：
文献类型：
--
作者：
Hill, J.;Gilbert, R.
目的牛重复繁殖综合征的发生与子宫内不良环境有关。亚临床子宫内膜炎是一个组成部分，因此，炎症影响胚胎存活的机制进行了研究，通过评估炎症子宫内膜的非细胞产物对胚胎发育的影响，以blastocyst.Design子宫内膜液从泌乳奶牛收集通过灌洗使用胚胎培养基。然后通过输注糖原诱导无菌性炎症，6 h后重复灌洗。回收的液体用于培养第5天体外衍生的胚胎2天。结果条件培养液和培养液+血清组的囊胚发育率高于对照组（炎症组42.2%，非炎症组49.3%，非炎症组50.9%，对照组16.9%，P < 0.001）。条件性炎症组的囊胚细胞数较低（炎症组，83.1;非炎症组，99.8;对照+血清组，100.6;对照组，110.1; P < 0.001）。条件性炎症组滋养外胚层细胞数减少，但内细胞团数无明显变化，内细胞团与滋养外胚层细胞数之比增加（P < 0.001）。内细胞团：滋养层比例增加可能会对胚胎存活产生负面影响。胚胎质量改变是亚临床肠炎奶牛早期胚胎失败的一种机制。用正常子宫内膜液培养胚胎可提高体外胚胎生产。
Objective A suboptimal uterine environment contributes to the bovine repeat breeder syndrome. Subclinical endometritis is a component, so the mechanism by which inflammation affects embryo survival was investigated by assessing the effect of non-cellular products of an inflamed endometrium on embryo development to blastocyst.Design Endometrial fluid from a lactating dairy cow was collected by lavage using embryo culture medium. Aseptic inflammation was then induced by infusion of glycogen and lavage was repeated 6 h later. The recovered fluid was used to culture Day 5 in-vitro-derived embryos for 2 days. Embryo development and quality were compared for two treatment groups (culture media conditioned by inflamed or non-inflamed endometrium) and two controls (control or control + serum).Results Development to blastocyst was higher for conditioned media or media + serum (inflamed 42.2%; non-inflamed 49.3%;- control + serum 50.9%; control 16.9%; P < 0.001). Blastocyst cell numbers were lower for the conditioned-inflamed group (inflamed, 83.1; non-inflamed, 99.8; control + serum, 100.6; control, 110.1; P < 0.001). Trophectoderm cell number, but not inner cell mass number, was reduced in the conditioned-inflamed group and the inner cell mass:trophectoderm ratio was increased (P < 0.001).Conclusion Altering the embryo culture environment with the products of endometrial inflammation had a subtle effect on embryo quality. An increased inner cell mass:trophoblast ratio is likely to negatively affect embryonic survival. Altered embryo quality is a mechanism for early embryonic failure in cows with subclinical endometritis. Culture of embryos with normal endometrial fluid may improve in vitro embryo production.