Nox Complex signal and MAPK cascade pathway are cross-linked and essential for pathogenicity and conidiation of mycoparasite Coniothyrium minitans.
Nox Complex signal and MAPK cascade pathway are cross-linked and essential for pathogenicity and conidiation of mycoparasite Coniothyrium minitans.
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Nox 复合物信号和 MAPK 级联途径是交联的，对于真菌寄生虫 Coniothyrium minitans 的致病性和分生孢子至关重要
DOI：
10.1038/srep24325
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发表时间：
2016-04-12
影响因子：
4.6
通讯作者：
Fu Y
中科院分区：
文献类型：
--
作者：
Wei W;Zhu W;Cheng J;Xie J;Jiang D;Li G;Chen W;Fu Y
鉴定了菌核菌寄生虫 Coniothyrium minitans 的 NADPH 氧化酶复合物，这是一种针对由核盘菌引起的农作物病害的重要生物防治剂，并研究了其在分生孢子和菌寄生中的功能。基因敲除和互补实验表明，CmNox1而非CmNox2是分生孢子和寄生所必需的，并且其表达可以被其宿主真菌显着诱导。 CmNox1 受 CmRac1-CmNoxR 调节，并与 CmSlt2 相互作用，CmSlt2 是编码细胞壁完整性相关 MAP 激酶的酿酒酵母 Slt2 的同源物。在ΔCmNox1中，CmSlt2-GFP融合蛋白失去了准确定位到细胞核的能力。过表达CmSlt2可以部分恢复ΔCmRac1分生孢子形成的缺陷，表明CmSlt2是CmNox1的下游调控因子，参与分生孢子形成和寄生。 CmNox1-CmSlt2信号通路基因敲除突变体在PDA上培养时，真菌寄生相关基因CmPks1、Cmg1和CH1的表达均受到抑制。因此，我们的研究推断CmRac1-CmNoxR通过调节CmNox1-CmSlt2途径来调节C. minitans的分生孢子和致病性。
The NADPH oxidase complex of a sclerotial mycoparasite Coniothyrium minitans, an important biocontrol agent against crop diseases caused by Sclerotinia sclerotiorum, was identified and its functions involved in conidiation and mycoparasitism were studied. Gene knock-out and complementary experiments indicated that CmNox1, but not CmNox2, is necessary for conidiation and parasitism, and its expression could be significantly induced by its host fungus. CmNox1 is regulated by CmRac1-CmNoxR and interacts with CmSlt2, a homolog of Saccharomyces cerevisiae Slt2 encoding cell wall integrity-related MAP kinase. In ΔCmNox1, CmSlt2-GFP fusion protein lost the ability to localize to the cell nucleus accurately. The defect of conidiation in ΔCmRac1 could be partially restored by over-expressing CmSlt2, indicating that CmSlt2 was a downstream regulatory factor of CmNox1 and was involved in conidiation and parasitism. The expressions of mycoparasitism-related genes CmPks1, Cmg1 and CH1 were suppressed in the knock-out mutants of the genes in CmNox1-CmSlt2 signal pathway when cultivated either on PDA. Therefore, our study infers that CmRac1-CmNoxR regulates CmNox1-CmSlt2 pathway in regulating conidiation and pathogenicity of C. minitans.