Expression of interleukin-10 and interleukin-12 in piglets experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV)
Expression of interleukin-10 and interleukin-12 in piglets experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV)
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DOI：
10.1016/s0021-9975(03)00036-7
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发表时间：
2003-08-01
影响因子：
0.8
通讯作者：
Chae, C
中科院分区：
文献类型：
--
作者：
Chung, HK;Chae, C
应用逆转录聚合酶链式反应和非放射性地高辛标记的c DNA探针原位杂交技术，研究了猪繁殖与呼吸综合征病毒(PRRSV)韩国分离株(北美株)经鼻腔接种福尔马林固定、石蜡包埋的仔猪肺组织中IL-10和IL-12基因的表达。IL-12p35阳性细胞在接种后第1天(Dpi)即可在肺内检测到，5dpi时阳性细胞数量增加，此后迅速减少。相反，IL-10和IL-12p40阳性细胞在1dpi时在肺内检测到，3dpi时它们的数量增加，此后迅速减少。IL-10、IL-12p35和IL-12p40的杂交信号总是与炎症有关。这些细胞因子在PRRSV感染仔猪的非皮损肺和对照仔猪的正常肺中的表达很低。肺组织连续切片原位杂交显示PRRSV和这些细胞因子在间质性肺炎中密切共存。提示IL-10和IL-12的表达在抗PRRSV感染的肺防御机制中起一定作用。(C)2003爱思唯尔有限公司。保留所有权利。
The expression of mRNA encoding interleukin-10 (IL-10) and IL-12 was studied, by the reverse transcription-polymerase chain reaction and by in-situ hybridization with a non-radioactive digoxigenin-labelled cDNA probe, in formalin-fixed, paraffin wax-embedded lung tissue from piglets inoculated intranasally with a Korean isolate (North American genotype) of porcine reproductive and respiratory syndrome virus (PRRSV). IL-12p35-positive cells were detected in the lung at I day post-inoculation (dpi), their number increasing at 5 dpi, and rapidly decreasing thereafter. In contrast, IL-10- and IL-12p40-positive cells were detected in the lung at 1 dpi, their number increasing at 3 dpi, and rapidly decreasing thereafter. Hybridization signals for IL-10, IL-12p35 and IL-12p40 were always associated with inflammation. Expression of these cytokines was minimal in non-lesional lung of PRRSV-infected piglets and in normal lung from control piglets. In-situ hybridization in serial sections of lung tissues indicated close co-localization of PRRSV and these cytokines in interstitial pneumonia. The results suggest that the expression of IL-10 and IL-12 plays a role in pulmonary defence mechanisms against PRRSV infection. (C) 2003 Elsevier Ltd. All rights reserved.