Fluorescence enhancement detection of underivatized amino acids using a trifluoroacetophenone-based tripodal fluoroionophore
Fluorescence enhancement detection of underivatized amino acids using a trifluoroacetophenone-based tripodal fluoroionophore
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DOI：
10.2533/000942905777676515
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发表时间：
2005
期刊：
影响因子：
1.2
通讯作者：
S. Sasaki;Gou Monma;D. Citterio;Koji Yamada;Koji Suzuki
中科院分区：
文献类型：
--
作者：
S. Sasaki;Gou Monma;D. Citterio;Koji Yamada;Koji Suzuki
合成了一种含有两个三氟苯乙酮基团和一个基团的六取代苯基的三脚架荧光载体，并考察了其作为荧光剂或离子交换膜或光学膜成分对几种未衍生化氨基酸的作用。在乙腈固-液萃取实验中，荧光增强反应的顺序为Phe、Cys、Gly。另一方面，聚氯乙烯聚合物膜在离子交换过程中表现出Phe的选择性和单阴离子能斯特响应，支持离子载体以阴离子形式与Phe结合的假设。结果表明，在流动体系中，基于该荧光载体的光学膜对苯丙氨酸或半胱氨酸具有荧光增强响应。
A tripodal fluoroionophore based on a hexasubstituted benzene unit having two trifluoroacetophenone groups and a pyrenyl moiety was synthesized, and its characteristics as fluorogenic reagent or component of an ISE or optode membrane towards several underivatized amino acids were examined. In a solid-liquid extraction experiment in acetonitrile, the fluorescence enhancement response was observed in the order of Phe, Cys, and Gly. On the other hand, the PVC-based polymeric membrane applied in ISE showed Phe selectivity with a monoanionic Nernstian response, supporting the assumption that the ionophore binds Phe in its anionic form. It is demonstrated that the optode membrane based on this fluoroionophore shows fluorescence enhancement response towards Phe or Cys in a flow-system.