A sensitive detection of phospho-Smad1/5/8 and Smad2 in Western blot analyses
A sensitive detection of phospho-Smad1/5/8 and Smad2 in Western blot analyses
复制标题
DOI：
10.1016/j.jbbm.2007.10.001
复制
发表时间：
2008-04-24
影响因子：
--
通讯作者：
Murakami, Masaru
中科院分区：
文献类型：
--
作者：
Funaba, Masayuki;Murakami, Masaru
转化生长因子-β家族参与多种生理过程，并通过受体复合体对Smad的磷酸化来传递信号。本研究探讨了蛋白质印迹分析中封闭液对磷酸化Smad1/5/8和Smad2检测的影响。当使用EzBlock作为封闭剂时，最有效地检测到磷酸化的Smad1/8和Smad2。抗磷酸化Smad2抗体特异性地识别Smad2的磷酸化形式，而抗磷酸化Smad1/5/8抗体也与非磷酸化形式发生反应。这些抗体与其他Smad不发生反应。(C)2007 Elsevier B.V.保留所有权利。
The transforming growth factor-beta (TGF-beta) family is involved in a variety of physiological processes, and transmits signals through phosphorylation of Smad by the receptor complexes. In the present study, effects of blocking solution in Western blot analyses on detection of phosphorylated Smad1/5/8 and Smad2 were examined. When EzBlock was used as a blocking reagent, phosphorylated Smad 1 /8 and Smad2 were most efficiently detected. The anti-phospho-Smad2 antibody specifically recognized the phosphorylated form of Smad2, whereas the antiphospho-Smad1/5/8 antibody also reacted to the unphosphorylated form. These antibodies did not react with the other Smads. (c) 2007 Elsevier B.V. All rights reserved.