Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters
Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters
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DOI：
10.1128/jb.173.14.4540-4543.1991
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发表时间：
1991-07
影响因子：
3.2
通讯作者：
M. Feldhaus;V. Hwa;Q. Cheng;A. Salyers
中科院分区：
文献类型：
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作者：
M. Feldhaus;V. Hwa;Q. Cheng;A. Salyers
我们已经构建了用于拟杆菌属的转录融合载体，在人类结肠中大量发现的一种革兰氏阴性专性厌氧菌。这些载体中的报告基团是来自大肠杆菌的无启动子的β-葡萄糖醛酸酶基因（uidA）。两种载体（pMJF-2和pMJF-3）在拟杆菌属中复制。第三个，pCQW-1，在拟杆菌属中不复制。并可用于引入E.大肠杆菌β-葡萄糖醛酸酶融合到拟杆菌染色体中。
We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.