Temporal and differential gene expression of Singapore grouper iridovirus
Temporal and differential gene expression of Singapore grouper iridovirus
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DOI：
10.1099/vir.0.82219-0
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发表时间：
2006-10-01
影响因子：
3.8
通讯作者：
Hew, Choy Leong
中科院分区：
文献类型：
--
作者：
Chen, Li Ming;Wang, Fan;Hew, Choy Leong
新加坡石斑鱼虹膜病毒（SGIV）是Ranavirus属虹膜病毒的一种，是造成石斑鱼养殖业重大经济损失的主要病原体。为了进一步研究其感染机制，研究人员首次为SGIV基因组构建了病毒DNA微阵列，以在体外同时测量其预测的开放阅读框的表达。利用病毒DNA芯片对SGIV的时间基因表达进行了表征，DNA芯片数据与实时RT-PCR研究结果一致。此外，通过结合药物治疗和DNA微阵列研究，发现了SGIV不同阶段的病毒基因（即即早、早、晚基因）。这些结果应该为虹膜病毒的复制和发病机制提供重要的见解。
Singapore grouper iridovirus (SGIV), an iridovirus in the genus Ranavirus, is a major pathogen that results in significant economic losses in grouper aquaculture. To investigate further its infective mechanisms, for the first time, a viral DNA microarray was generated for the SGIV genome to measure the expression of its predicted open reading frames simultaneously in vitro. By using the viral DNA microarray, the temporal gene expression of SGIV was characterized and the DNA microarray data were consistent with the results of real-time RT-PCR studies. Furthermore, different-stage viral genes (i.e. immediate-early, early and late genes) of SGIV were uncovered by combining drug treatments and DNA microarray studies. These results should offer important insights into the replication and pathogenesis of iridoviruses.