Pathobiology of triple reassortant H3N2 influenza viruses in breeder turkeys and its potential implication for vaccine studies in turkeys
Pathobiology of triple reassortant H3N2 influenza viruses in breeder turkeys and its potential implication for vaccine studies in turkeys
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DOI：
10.1016/j.vaccine.2008.11.076
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发表时间：
2009-02-05
期刊：
影响因子：
5.5
通讯作者：
Lee, C. -W.
中科院分区：
文献类型：
--
作者：
Pillai, S. P. S.;Pantin-Jackwood, M.;Lee, C. -W.
自2003年以来，美国已从火鸡中分离出三重重组H3N2流感病毒。这些TR H3N2病毒感染与种鸡产蛋量急剧下降有关，尽管多种病原体的共同感染可能会加剧临床症状。在这项研究中，我们通过实验证实，trh3n2流感病毒单独可以导致26周龄种鸡的产蛋量急剧减少或完全停止，并导致生殖道病理。在这些火鸡的输卵管中，我们证实了高水平的病毒复制和鸟类特异性α 2,3唾液酸-半乳糖受体的大量分布。虽然2-6周大的火鸡通常用于致病性和疫苗保护研究，但这一年龄组的病毒脱落水平低和无症状感染常常给解释结果带来困难。我们的研究表明，应该用种鸡来评估TR H3N2病毒的潜在致病性，在疫苗功效研究中，病毒滴度和输卵管病理以及产蛋数据可以作为体内攻击后的良好保护措施。(C) 2008 Elsevier Ltd版权所有，
Triple reassortant (TR) H3N2 influenza viruses have been isolated from turkeys in the United States since 2003. These TR H3N2 virus infections have been associated with drastic declines in egg production in breeder turkeys although co-infection with multiple agents could have been responsible for exacerbating the clinical signs. In this study, we experimentally confirmed that TR H3N2 influenza virus alone can cause drastic reduction/complete cessation of egg production and pathology of the reproductive tract in 26-week-old breeder turkeys. We confirmed high levels of virus replication and abundant distribution Of avian specific alpha 2,3 sialic acid-galactose receptors ill the oviduct of these turkeys. Although 2-6-week-old turkeys are routinely used for pathogenicity and vaccine protection studies, the low levels of viral shedding and asymptomatic infections in this age group often pose difficulty in interpretation of results. Our study shows that breeder turkeys Should be used to assess the potential pathogenicity of TR H3N2 viruses and the viral titers and pathology of the oviduct as well as egg production data can be good measures of protection following in vivo challenge in vaccine efficacy Studies. (C) 2008 Elsevier Ltd. All rights reserved,