Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells.
Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells.
复制标题
小鼠角质形成细胞和皮肤免疫细胞亚群的流式细胞术分析。
DOI：
10.1016/j.xpro.2021.101052
复制
发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Nagao K
中科院分区：
文献类型：
--
作者：
Sakamoto K;Goel S;Funakoshi A;Honda T;Nagao K
皮肤是我们身体最外面的物理屏障，是一个免疫界面，富含各种免疫和非免疫细胞。然而，高效地产生用于流式细胞术分析的单细胞悬液可能是具有挑战性的。在这里，我们提供了获得表皮和整个皮肤细胞悬液的方案，以及通过流式细胞术鉴定小鼠角质形成细胞和皮肤免疫细胞亚群的门控策略。有关本议定书的使用和执行的完整细节，请参阅。小鼠表皮单细胞悬液的制备及全皮肤抗体染色用于角质形成细胞、ILC和髓系细胞的流式细胞术门控策略角质形成细胞、ILC和髓系细胞亚群的门控策略皮肤是我们身体最外层的物理屏障和免疫界面，富含各种免疫和非免疫细胞。然而，高效地产生用于流式细胞术分析的单细胞悬液可能是具有挑战性的。在这里，我们提供了获得表皮和整个皮肤细胞悬液的方案，以及通过流式细胞术鉴定小鼠角质形成细胞和皮肤免疫细胞亚群的门控策略。
Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to. Preparation of single-cell suspension from mouse epidermis and whole skin Antibody staining for keratinocytes, ILCs, and myeloid cells for flow cytometry Gating strategy for keratinocyte, ILC, and myeloid cell subsets Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry.