Flow cytometry increases the sensitivity of detection of leukemia and lymphoma cells in bronchoalveolar lavage specimens.
Flow cytometry increases the sensitivity of detection of leukemia and lymphoma cells in bronchoalveolar lavage specimens.
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流式细胞术提高了支气管肺泡灌洗标本中白血病和淋巴瘤细胞检测的灵敏度。
DOI：
10.1002/cyto.b.21033
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yuan,ConstanceM
中科院分区：
文献类型：
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作者：
Song,JooY;Filie,ArmandoC;Venzon,David;Stetler-Stevenson,Maryalice;Yuan,ConstanceM
研究背景近年来的研究证实，流式细胞术（FC）在检测血淋巴细胞肿瘤（HLN）中的敏感性明显高于细胞形态学（CM）。然而，它的效用在少细胞支气管肺泡灌洗（BAL）specimens尚未established.MethodsFC从44例HLN的诊断前提交的BAL标本。根据标本的细胞结构和患者病史选择样本组。FC的结果进行了比较，同时CM evaluations.ResultsAll 44巴尔斯被认为是令人满意的FC，并产生了翔实的结果，有助于诊断。诊断包括22/44例B细胞肿瘤、16/44例T细胞肿瘤、4/44例骨髓肿瘤和2/44例浆细胞肿瘤。在77%（34/44）的病例中证实了FC和CM之间的总体一致性。在9/44例病例（20%）中，一种技术（FC或CM）清楚地检测到恶性细胞，而另一种则没有。在8/9例不一致病例中，FC在检测HLN方面比CM更敏感。只有一种情况下（1/44，2%）是恶性HLN细胞怀疑CM，但没有确定FC（1/44，2%）。FC适用于HLN的BAL评估，并且相对于单独的CM提高了HLN检测的灵敏度。在BAL的诊断评价中，FC和CM的综合方法优于单独的任何一种技术上级。出版社：Wiley Periodicals，Inc.
BackgroundRecent studies have definitively determined that flow cytometry (FC) is significantly more sensitive than cytomorphology (CM) in detection of hematolymphoid neoplasms (HLNs). However, its utility in paucicellular bronchoalveolar lavage (BAL) specimens has not been established.MethodsFC was performed on BAL specimens submitted from 44 patients with a prior diagnosis of HLN. Panels chosen were based upon cellularity of specimen and patient history. FC results were compared with concurrent CM evaluations.ResultsAll 44 BALs were deemed satisfactory for FC and yielded informative results that assisted in diagnosis. Diagnoses included 22/44 B‐cell neoplasms, 16/44 T‐cell neoplasms, four/44 myeloid neoplasms, and two/44 plasma cell neoplasms. Overall concordance was demonstrated between FC and CM in 77% (34/44) of cases. In nine/44 cases (20%), one technique (FC or CM) clearly detected malignant cells when the other did not. FC was more sensitive than CM in detecting a HLN in eight/nine discordant cases. In only one case (one/44, 2%) were malignant HLN cells suspected by CM, but not identified by FC (one/44, 2%).ConclusionWe demonstrate, in the largest series published to date, that FC can be performed on BAL specimens. FC is indicated in evaluation of BAL for HLN and improves sensitivity of detection of HLN over CM alone. An integrated FC and CM approach is superior to either technique alone in diagnostic evaluation of BAL. Published 2012 Wiley Periodicals, Inc.