Immune cell quantitation in normal breast tissue lobules with and without lobulitis
Immune cell quantitation in normal breast tissue lobules with and without lobulitis
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DOI：
10.1007/s10549-014-2896-8
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发表时间：
2014-04-01
影响因子：
3.8
通讯作者：
Visscher, Daniel W.
中科院分区：
文献类型：
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作者：
Degnim, Amy C.;Brahmbhatt, Rushin D.;Visscher, Daniel W.
虽然免疫微环境在乳腺癌中得到了研究，但对其在非恶性乳腺组织中的作用却知之甚少。在这里，我们对正常乳腺组织小叶中的细胞免疫成分进行量化和定位，无论是否存在可见的免疫浸润（小叶炎）。对 11 个正常乳腺组织样本中的最多 10 个代表性小叶进行了 B 细胞 (CD20)、细胞毒性 T 细胞 (CD8)、辅助 T 细胞 (CD4)、树突状细胞 (CD11c)、白细胞 (CD45) 和单核细胞/巨噬细胞 (CD68) 的评估。使用数字图像分析，测量并比较有/无小叶炎的小叶之间的免疫细胞密度。对 11 个正常乳腺组织样本中的 109 个小叶进行了评估； 31 例患有小叶炎，78 例没有小叶炎。免疫细胞在所有正常样本中表现出一致的模式，主要位于小叶而不是间质。无论小叶炎状态如何，大多数小叶均显示 CD8+、CD11c+、CD45+ 和 CD68+ 细胞，CD4+ 和 CD20+ 细胞密度较低。 CD11c+ 和 CD8+ 细胞均与小叶上皮的基底部分一致且密切相关。在患有小叶炎的小叶中观察到 CD4+、CD8+、CD20+ 和 CD45+ 细胞的密度显着较高。相反，单核细胞/巨噬细胞和树突状细胞的密度不随小叶炎而变化。在正常乳腺组织中，存在髓样细胞和淋巴样细胞并定位于小叶，细胞毒性 T 细胞和树突状细胞直接与上皮结合。患有小叶炎的小叶具有明显更多的适应性免疫（B 和 T）细胞，但树突状细胞或单核细胞/巨噬细胞没有增加。这些发现表明正常乳腺组织中存在活跃且动态的粘膜免疫系统。
While the immune microenvironment has been investigated in breast cancers, little is known about its role in non-malignant breast tissues. Here we quantify and localize cellular immune components in normal breast tissue lobules, with and without visible immune infiltrates (lobulitis). Up to ten representative lobules each in eleven normal breast tissue samples were assessed for B cells (CD20), cytotoxic T cells (CD8), helper T cells (CD4), dendritic cells (CD11c), leukocytes (CD45), and monocytes/macrophages (CD68). Using digital image analysis, immune cell densities were measured and compared between lobules with/without lobulitis. 109 lobules in 11 normal breast tissue samples were evaluated; 31 with lobulitis and 78 without. Immune cells showed consistent patterns in all normal samples, predominantly localized to lobules rather than stroma. Regardless of lobulitis status, most lobules demonstrated CD8+, CD11c+, CD45+, and CD68+ cells, with lower densities of CD4+ and CD20+ cells. Both CD11c+ and CD8+ cells were consistently and intimately associated with the basal aspect of lobule epithelium. Significantly higher densities of CD4+, CD8+, CD20+, and CD45+ cells were observed in lobules with lobulitis. In contrast, densities of monocytes/macrophages and dendritic cells did not vary with lobulitis. In normal breast tissue, myeloid and lymphoid cells are present and localized to lobules, with cytotoxic T and dendritic cells directly integrated with epithelium. Lobules with lobulitis have significantly more adaptive immune (B and T) cells, but no increase in dendritic cells or monocytes/macrophages. These findings indicate an active and dynamic mucosal immune system in normal breast tissue.