Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Aral 1957) corrig., sp nov., nom. rev.
Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Aral 1957) corrig., sp nov., nom. rev.
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DOI：
10.1099/ijs.0.02238-0
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发表时间：
2003-09-01
影响因子：
2.8
通讯作者：
Kasai, H
中科院分区：
文献类型：
--
作者：
Hatano, K;Nishii, T;Kasai, H
重新评估了 64 个轮生链霉菌（以前称为链轮丝菌）菌株的分类地位，并根据菌株的表型、DNA-DNA 杂交数据和 gyrB（DNA 旋转酶 B 亚基的结构基因）的部分序列对菌株进行了重新分类。这些菌株由有效公布名称的46种和8个亚种以及未有效公布名称的13种组成[其中包括国际链霉菌计划(ISP)审查的10株]，根据其表型和gyrB基因序列分为典型和非典型轮生链霉菌属两类。根据97％gyrB序列相似性的阈值，将典型的轮生物种（59株）分为三个或更多物种的6个大簇，两个物种的7个小簇和5个单成员簇。主要菌群的典型是Streptomyces abikoensis、Streptomyces cinnamoneus、Streptomyces farlicus、Streptomyces griseocarneus、Streptomyces hiroshimensis和Streptomyces netropsis。在表型上，每个簇的成员彼此非常相似，除了S.distallicus簇和S.netropsis簇之外，S.distallicus簇在表型上分为S.distallicus和Streptomyces stramineus亚簇，而S.netropsis簇则分为S.netropsis和Streptomyces eurocidicus亚簇。每个小簇中的菌株在表型上彼此非常相似。每个主要簇和/或亚簇中的代表性物种与其他物种之间以及次要簇中的菌株之间的 DNA-DNA 相关性 > 70%，表明主要簇和/或亚簇以及次要簇各自包含单个物种。结果表明，59株典型轮生链霉菌属18种，包括主观异名S. abikoensis、Streptomyces ardus、Streptomycesblastmyceticus、S.cinnamoneus、S.eurocidicus、S.griseocarneus、S.hiroshimensis、Streptomyces lilacinus、‘Streptomyces utereticuli’、链霉菌属 黄孢子菌、马须链霉菌、茂原链霉菌、莫鲁卡链霉菌、S. netropsis、Streptomyces orinoci、S. stramineus、硫黄链霉菌和绿色链霉菌。
The taxonomic status of 64 strains of whorl-forming Streptomyces (formerly Streptoverticillium) species was re-evaluated and strains were reclassified on the basis of their phenotypes, DNA-DNA hybridization data and partial sequences of gyrB, the structural gene of the B subunit of DNA gyrase. These strains, which consisted of 46 species and eight subspecies with validly published names and 13 species whose names have not been validly published [including 10 strains examined by the International Streptomyces Project (ISP)], were divided into two groups, namely typical and atypical whorl-forming Streptomyces species, based on their phenotypes and gyrB gene sequences. The typical whorl-forming species (59 strains) were divided into six major clusters of three or more species, seven minor clusters of two species and five single-member clusters, based on the threshold value of 97 % gyrB sequence similarity. Major clusters were typified by Streptomyces abikoensis, Streptomyces cinnamoneus, Streptomyces distallicus, Streptomyces griseocarneus, Streptomyces hiroshimensis and Streptomyces netropsis. Phenotypically, members of each cluster resembled each other closely except for the S. distallicus cluster, which was divided phenotypically into the S. distallicus and Streptomyces stramineus subclusters, and the S. netropsis cluster, which was divided into the S. netropsis and Streptomyces eurocidicus subclusters. Strains in each minor cluster closely resembled each other phenotypically. DNA-DNA relatedness between the representative species and others in each major cluster and/or subcluster, and between strains in the minor clusters, was > 70 %, indicating that the major clusters and/or subclusters and the minor clusters each comprise a single species. It was concluded that 59 strains of typical whorl-forming Streptomyces species consisted of the following 18 species, including subjective synonym S. abikoensis, Streptomyces ardus, Streptomyces blastmyceticus, S. cinnamoneus, S. eurocidicus, S. griseocarneus, S. hiroshimensis, Streptomyces lilacinus, 'Streptomyces luteoreticuli', Streptomyces luteosporeus, Streptomyces mashuensis, Streptomyces mobaraensis,Streptomyces morookaense, S. netropsis, Streptomyces orinoci, S. stramineus, Streptomyces thioluteus and Streptomyces viridiflavus.