Single-cell transcriptome provides novel insights into antler stem cells, a cell type capable of mammalian organ regeneration
Single-cell transcriptome provides novel insights into antler stem cells, a cell type capable of mammalian organ regeneration
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DOI：
10.1007/s10142-019-00659-2
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发表时间：
2019-07-01
影响因子：
2.9
通讯作者：
Li, Chunyi
中科院分区：
文献类型：
--
作者：
Ba, Hengxing;Wang, Datao;Li, Chunyi
鹿茸再生是一种基于干细胞的表型过程，有可能成为再生医学的一个有价值的模型。鹿茸干细胞（ASC）库位于生茸骨膜（AP）中，用于鹿茸发育。然而，这个ASC池是同质的还是异质的尚未得到充分评价。在这项研究中，我们基于10 x Genomics平台（scRNA-seq）在单细胞水平上为ASC生成了一个全面的转录组数据集。总共4565个ASC被测序并分类成一个大的细胞簇，表明AP中的ASC可能是一个同质群体。scRNA-seq数据显示，肿瘤相关基因在这些同质ASC中高度表达，即，TIMP1、TMSB 10、LGALS 1、FTH 1、Vim、LOC 110126017和S100 A4。干细胞标记物筛选结果表明，ASCs可能被认为是介于胚胎干细胞（CD 9）和成体干细胞（CD 29、CD 90、NPM 1和Vim）之间的一种特殊类型的干细胞。我们的研究结果在单细胞水平上为ASC提供了第一个全面的转录组分析，并确定了AP中仅有一种主要的细胞类型和一些关键的干细胞基因，这可能是为什么鹿角这种独特的哺乳动物器官一旦丢失就可以完全再生的关键。
Antler regeneration, a stem cell-based epimorphic process, has a potential as a valuable model for regenerative medicine. A pool of antler stem cells (ASCs) for antler development is located in the antlerogenic periosteum (AP). However, whether this ASC pool is homogenous or heterogeneous has not been fully evaluated. In this study, we produced a comprehensive transcriptome dataset at the single-cell level for the ASCs based on the 10x Genomics platform (scRNA-seq). A total of 4565 ASCs were sequenced and classified into a large cell cluster, indicating that the ASC resident in the AP are likely to be a homogeneous population. The scRNA-seq data revealed that tumor-related genes were highly expressed in these homogeneous ASCs, i.e., TIMP1, TMSB10, LGALS1, FTH1, VIM, LOC110126017, and S100A4. Results of screening for stem cell markers suggest that the ASCs may be considered as a special type of stem cell between embryonic (CD9) and adult (CD29, CD90, NPM1, and VIM) stem cells. Our results provide the first comprehensive transcriptome analysis at the single-cell level for the ASCs and identified only one major cell type resident in the AP and some key stem cell genes, which may hold the key to why antlers, the unique mammalian organ, can fully regenerate once lost.