Random Walk of Processive, Quantum Dot-Labeled Myosin Va Molecules within the Actin Cortex of COS-7 Cells
Random Walk of Processive, Quantum Dot-Labeled Myosin Va Molecules within the Actin Cortex of COS-7 Cells
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DOI：
10.1016/j.bpj.2009.04.052
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发表时间：
2009-07-22
影响因子：
3.4
通讯作者：
Warshaw, David M.
中科院分区：
文献类型：
--
作者：
Nelson, Shane R.;Ali, M. Yusuf;Warshaw, David M.
肌球蛋白Va（myoVa）是一种基于肌动蛋白的细胞内货物转运蛋白。体外实验已经证实，单个myoVa沿着沿着肌动蛋白轨道向前运动，但对这种马达在细胞内如何运作知之甚少。在这里，我们使用全内反射荧光显微镜跟踪量子点（Qdot）标记的myoVa HMM在COS-7细胞中的运动。这种标记方法是独特的，因为它允许跟踪myoVa，而不是其货物。单颗粒分析显示，短时间内（
Myosin Va (myoVa) is an actin-based intracellular cargo transporter. In vitro experiments have established that a single myoVa moves processively along actin tracks, but less is known about how this motor operates within cells. Here we track the movement of a quantum dot (Qdot)-labeled myoVa HMM in COS-7 cells using total internal reflectance fluorescence microscopy. This labeling approach is unique in that it allows myoVa, instead of its cargo, to be tracked. Single-particle analysis showed short periods (