IMIPENEM RESISTANCE IN PSEUDOMONAS-AERUGINOSA RESULTING FROM DIMINISHED EXPRESSION OF AN OUTER-MEMBRANE PROTEIN
IMIPENEM RESISTANCE IN PSEUDOMONAS-AERUGINOSA RESULTING FROM DIMINISHED EXPRESSION OF AN OUTER-MEMBRANE PROTEIN
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DOI：
10.1128/aac.31.5.703
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发表时间：
1987-05-01
影响因子：
4.9
通讯作者：
OPFERKUCH, W
中科院分区：
文献类型：
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作者：
BUSCHER, KH;CULLMANN, W;OPFERKUCH, W
研究了5株亚胺培南敏感和耐药菌株铜绿假单胞菌对亚胺培南的耐药机制。亚胺培南耐药变异的选择频率从2.7 X 10（-5）到2.1 X 10（-8）不等，与其他β -内酰胺的选择频率相当。亚胺培南与其他β -内酰胺类化合物间未见交叉耐药。在所有亚胺培南耐药变异中，亚胺培南对染色体β -内酰胺酶的诱导作用与敏感亲本菌株相比明显减弱。其他诱导剂如氨苄西林或头孢西丁则不是这种情况，这表明亚胺培南的吸收受损是耐药性的一个解释。外膜蛋白分析显示，46千道尔顿或45千道尔顿蛋白明显减少。耐亚胺培南变异体的外膜脂多糖没有改变。
The mechanism of Pseudomonas aeruginosa resistance to imipenem in five imipenem-susceptible clinical isolates and in their resistant counterparts was investigated. The frequency for selecting imipenem-resistant variants ranged from 2.7 X 10(-5) to 2.1 X 10(-8) and was comparable to those for other beta-lactams. Cross-resistance between imipenem and other beta-lactam compounds was not observed. In all imipenem-resistant variants, induction of chromosomal beta-lactamase by imipenem was markedly diminished compared with that in the susceptible parent strain. This was not the case for other inducers such as ampicillin or cefoxitin, suggesting an impaired uptake of imipenem as an explanation for resistance. Analysis of the outer membrane proteins revealed a marked decrease of either a 46- or a 45-kilodalton protein. The lipopolysaccharide of the outer membrane in the imipenem-resistant variants was not altered.