Characterization of intracellular reverse transcription complexes of Moloney murine leukemia virus
Characterization of intracellular reverse transcription complexes of Moloney murine leukemia virus
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DOI：
10.1128/jvi.73.11.8919-8925.1999
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发表时间：
1999-11-01
影响因子：
5.4
通讯作者：
Goff, SP
中科院分区：
文献类型：
--
作者：
Fassati, A;Goff, SP
为了研究Moloney小鼠白血病病毒(MoMLV)生命周期的早期事件，我们分析了介导反转录的细胞内复合体。通过平衡密度分级和速度沉降法对逆转录复合体(RTCs)进行部分纯化，结果表明在细胞感染后不久形成了三种不同的胞内复合体。这些物种中只有一个能够在细胞质中启动和完成逆转录，这种RTC至少由病毒基因组、衣壳、整合酶和逆转录酶蛋白组成。RTC对微球菌核酸酶具有渗透性，但对抗体不具有渗透性。逆转录开始后不久，RTC内的病毒强STOP DNA就会受到保护，不会被核酸酶消化。在逆转录过程中，RTC的沉降速度减慢。进入细胞核后，大部分衣壳蛋白从RTC中消失，其沉降速度进一步减慢。
To examine the early events in the life cycle of Moloney murine leukemia virus (MoMLV), we analyzed the intracellular complexes mediating reverse transcription. Partial purification of the reverse transcription complexes (RTCs) by equilibrium density fractionation and velocity sedimentation indicated that three distinct species of intracellular complexes are formed shortly after cell infection. Only one of these species is able to start and complete reverse transcription in the cell cytoplasm, This RTC is composed of at least the viral genome, capsid, integrase, and reverse transcriptase proteins. The RTC becomes permeable to micrococcal nuclease but not to antibodies. Shortly after initiation of reverse transcription, the viral strong stop DNA within the RTC is protected from nuclease digestion. The sedimentation velocity of the RTC decreases during reverse transcription. After entry into the nucleus, most capsid proteins are lost from the RTC and its sedimentation velocity decreases further.