Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21:: a randomized, double-blind, placebo-controlled study
Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21:: a randomized, double-blind, placebo-controlled study
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DOI：
10.1111/j.1600-051x.2008.01306.x
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发表时间：
2008-10-01
影响因子：
6.7
通讯作者：
Hirata, Haruhisa
中科院分区：
文献类型：
--
作者：
Shimauchi, Hidetoshi;Mayanagi, Gen;Hirata, Haruhisa
目的：评价益生菌干预对非重度牙周炎志愿者牙周状况的影响。材料与方法：采用随机双盲法，给志愿者口服含唾液乳杆菌WB21(WB21)冻干片或安慰剂。最终，66名志愿者被随机分配到含有WB21(6.7×10(8)CFU)和木糖醇(安慰剂)的片剂中，每天三次，共8周。分别在基线(BL)、4周和干预结束时(8周)采集牙周临床参数和全唾液样本。采用酶联免疫吸附试验检测唾液乳铁蛋白(LF)水平。结果：干预8周后，两组患者的牙周临床参数均有改善。与安慰剂组相比，试验组中当前吸烟者的斑块指数和从BL到探查口袋深度的改善明显更大。试验组吸烟者唾液LF水平也显著降低。结论：益生菌可能有助于改善/维持牙周病高危人群的口腔健康。
Aim: This randomized clinical study was designed to evaluate the effect of probiotic intervention using lactobacilli on the periodontal condition of volunteers without severe periodontitis.Materials and Methods: Freeze-dried Lactobacillus salivarius WB21 (WB21)-containing tablets or a placebo were given to volunteers in a double-blind randomized study. A total of 66 volunteers were finally enrolled and randomly assigned to receive tablets containing WB21 (6.7 x 10(8) CFU) with xylitol or xylitol alone (placebo) three times a day for 8 weeks. Periodontal clinical parameters and whole saliva samples were obtained at baseline (BL), 4 weeks, and the end of the interventional period (8 weeks). Salivary lactoferrin (Lf) levels were measured by enzyme-linked immunosorbent assay. Lactobacilli in saliva and plaque samples was detected by semi-quantitative RT-PCR using 16S rRNA primers.Results: Periodontal clinical parameters were improved in both groups after an 8-week intervention. Current smokers in the test group showed a significantly greater improvement of plaque index and probing pocket depth from BL when compared with those in the placebo group. Salivary Lf level was also significantly decreased in the test group smokers.Conclusion: Our results indicate that probiotics could be useful in the improvement/maintenance of oral health in subjects at a high risk of periodontal disease.