Excretion of malondialdehyde, formaldehyde, acetaldehyde and acetone in the urine of rats following acute and chronic administration of ethanol.
Excretion of malondialdehyde, formaldehyde, acetaldehyde and acetone in the urine of rats following acute and chronic administration of ethanol.
复制标题
急性和慢性服用乙醇后大鼠尿液中丙二醛、甲醛、乙醛和丙酮的排泄。
作者：
S. File;J. Moser;D. Bagchi;P.l. Akubue;S. Stohs
最近的研究表明，引起氧化应激的外来物质会导致大鼠尿液中乙醛(ACT)、甲醛(FA)、丙酮(ACON)和丙二醛(MDA)的产生和排泄增加。因此，我们研究了急性和慢性乙醇给药对雌性SD大鼠这四种脂代谢产物排泄的影响。在干冰上收集尿样6小时。尿液用2，4-二硝基苯肼盐酸盐衍生，正戊烷萃取。采用高效液相色谱(HPLC法)对4种脂类代谢产物中的酰肼类化合物进行定量。在一次性口服5g乙醇/公斤的急性剂量后，ACT的尿液排泄量在治疗后6至12小时内增加了约5.8倍，此后又下降。从0到12小时，FA的排泄量减少了大约50%，在18-24小时的尿样中恢复到对照组的值，在42-48小时比对照组的值大1.3倍。从0小时到30小时，ACON比对照组增加了3.1倍，并在研究的其余18小时一直保持在升高水平。从18小时到36小时，丙二醛的排泄量增加了约1.5倍，然后在48小时的时间点保持不变。在单独的一系列实验中，大鼠连续10天长期口服0.5g乙醇/公斤，并从注射乙醇的第一天开始，连续11天检测尿脂代谢产物丙二醛、脂肪酸、ACT和ACON的排泄。
Recent studies have shown that xenobiotics which induce oxidative stress result in an increased production and excretion of acetaldehyde (ACT), formaldehyde (FA), acetone (ACON) and malondialdehyde (MDA) in the urine of rats. We have therefore examined the effect of acute and chronic ethanol administration on the excretion of these four lipid metabolites in female Sprague-Dawley rats. Urine samples were collected over dry ice for 6 hr time periods. Aliquots of urine were derivatized with 2,4-dinitrophenylhydrazine HCl, and extracted with n-pentane. High pressure lipid chromatogrpahy (HPLC) was used to quantitate and the hydrazones of the four lipid metabolite products. Following a single, oral, acute dose of 5 g ethanol/kg, urinary excretion of ACT increased approximately 5.8-fold from 6 to 12 hr posttreatment, and decreased thereafter. FA excretion decreased by approximately 50% from 0 to 12 hr, returned to control values in the 18-24 hr urine samples, and was 1.3-fold greater than control values at 42-48 hr. ACON increased 3.1-fold over control values from 0 to 30 hr and remained elevated throughout the remaining 18 hr of the study. The excretion of MDA increased approximately 1.5-fold from 18 to 36 hr, then remained constant through the 48 hr time point. In a separate series of experiments, a chronic oral dose of 0.5 g ethanol/kg was administered to rats for 10 consecutive days and the urinary excretion of the lipid metabolites MDA, FA, ACT and ACON was examined for 11 days, beginning with the first day of ethanol administration.(ABSTRACT TRUNCATED AT 250 WORDS)