Novel extracellular matrix architecture on excitatory neurons revealed by HaloTag-HAPLN1.
Novel extracellular matrix architecture on excitatory neurons revealed by HaloTag-HAPLN1.
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HaloTag-HAPLN1 揭示了兴奋性神经元的新型细胞外基质结构。
DOI：
10.1101/2024.03.29.587384
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Park,Sungjin
中科院分区：
文献类型：
--
作者：
Sterin,Igal;Niazi,Ava;Kim,Jennifer;Park,Joosang;Park,Sungjin
脑的细胞外基质（ECM）调节神经元的可塑性和动物的行为。ECM染色显示神经元子集周围呈网状结构的聚集模式，以及间质基质中的弥漫染色。然而，了解ECM沉积在各种神经元类型和亚细胞区室的结构特征仍然有限。为了可视化透明质酸支架ECM在大脑中的组织模式和组装过程，我们将HaloTag融合到HAPLN1，HAPLN1连接透明质酸和蛋白聚糖。该探针的表达或应用使我们能够确定ECM沉积的空间和时间调节以及神经元群体之间ECM聚集的异质性。双色birthdating显示ECM组装过程中的文化和在体内。在体内稀疏表达揭示了兴奋性神经元和发育调节的树突状ECM周围的ECM结构的新形式。总的来说，我们的研究揭示了大脑ECM的广泛结构特征，表明在调节神经元可塑性方面的不同作用。
The brain’s extracellular matrix (ECM) regulates neuronal plasticity and animal behavior. ECM staining shows an aggregated pattern in a net-like structure around a subset of neurons and diffuse staining in the interstitial matrix. However, understanding the structural features of ECM deposition across various neuronal types and subcellular compartments remains limited. To visualize the organization pattern and assembly process of the hyaluronan-scaffolded ECM in the brain, we fused a HaloTag to HAPLN1, which links hyaluronan and proteoglycans. Expression or application of the probe enables us to identify spatial and temporal regulation of ECM deposition and heterogeneity in ECM aggregation among neuronal populations. Dual-color birthdating shows the ECM assembly process in culture and in vivo. Sparse expression in vivo reveals novel forms of ECM architecture around excitatory neurons and developmentally regulated dendritic ECM. Overall, our study uncovers extensive structural features of the brain’ ECM, suggesting diverse roles in regulating neuronal plasticity.