A MURINE EARLY THYMOCYTE DEVELOPMENTAL SEQUENCE IS MARKED BY TRANSIENT EXPRESSION OF THE INTERLEUKIN-2 RECEPTOR
A MURINE EARLY THYMOCYTE DEVELOPMENTAL SEQUENCE IS MARKED BY TRANSIENT EXPRESSION OF THE INTERLEUKIN-2 RECEPTOR
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DOI：
10.1073/pnas.86.5.1614
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发表时间：
1989-03-01
影响因子：
11.1
通讯作者：
SCOLLAY, R
中科院分区：
文献类型：
--
作者：
PEARSE, M;WU, L;SCOLLAY, R
所有 T 谱系细胞的前体都存在于缺乏 CD4 和 CD8 表面标记的胸腺细胞群中。这些“双阴性”胸腺细胞是异质的，可以根据其其他表面标志物的表达分为离散的亚群。我们根据 T 细胞受体基因的重排和表达程度、细胞周期状态以及胸腺重建能力确定了这些亚群的相对成熟度。在表达高水平热稳定抗原的双阴性亚群中，附加标记吞噬糖蛋白 1 (Pgp-1) 和白细胞介素 2 受体 (IL-2R) 可用于定义序列 IL-2R-Pgp-1+ .fwdarw。 IL-2R+ Pgp-1-.fwdarw。 IL-2R-Pgp-1-，发生在CD4和CD8表达之前。 IL-2R 的瞬时表达标志着细胞增殖爆发和胸腺重建能力丧失之前序列中的一个重要发育点。该序列中最早的细胞已经针对 C.beta.1 区域中的基因进行了部分重排。 IL-2R表达标志着T细胞抗原受体β链基因重排的第二波以及T细胞抗原受体α和β链基因表达的开始。
Precursors of all T-lineage cells are found in the population of thymocytes that lacks the CD4 and CD8 surface markers. These "double-negative" thymocytes are heterogeneous and can be divided into discrete subpopulation based on their expression of other surface markers. We have determined the relative maturity of these subpopulations based on the extent of rearrangement and expression of their T-cell receptor genes, their cell cycle status, and their thymus reconstitution capacity. Within the subpopulation of double negatives expressing high levels of the heat-stable antigen, the additional markers phagocytic glycoprotein 1 (Pgp-1) and interleukin 2 receptor (IL-2R) can be used to define the sequence IL-2R- Pgp-1+ .fwdarw. IL-2R+ Pgp-1- .fwdarw. IL-2R- Pgp-1-, which occurs before the expression of CD4 and CD8. Transient expression of the IL-2R marks an important developmental point in the sequence just prior to a burst of cell proliferation and a loss of thymus reconstitution ability. The earliest cells in this sequence are already partially rearranged for genes in the C.beta.1 region. IL-2R expression marks a second wave of T-cell antigen receptor of .beta.-chain gene rearragement and the initiation of T-cell antigen receptor .alpha.- and .beta.-chain gene expression.