Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells
Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells
复制标题
DOI：
10.1002/cbin.10652
复制
发表时间：
2016-10-01
影响因子：
3.9
通讯作者：
Nishimura, Fusanori
中科院分区：
文献类型：
--
作者：
Hashimoto, Yoko;Kobayashi, Mari;Nishimura, Fusanori
在这项研究中，我们研究了参与Wnt信号鞘氨醇-1-磷酸（S1 P）增强成骨分化的C3 H10 T1/2多能干细胞。我们发现，S1 P增强Wnt 5a和低密度脂蛋白受体相关蛋白5或6（LRP 5/6）在成骨分化的表达。Wnt 5a中和抗体抑制S1 P增强的LRP 5/6和碱性磷酸酶的表达，这是成骨分化所必需的。相反，S1 P不影响内源性经典Wnt信号传导。总之，S1 P增强的Wnt 5a促进LRP 5/6表达，导致C3 H10 T1/2细胞成骨分化的触发。这些发现表明S1 P在骨再生中具有潜在的有益作用。
In this study, we investigated the involvement of Wnt signaling in sphingosine-1-phosphate (S1P)-enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low-density lipoprotein receptor-related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a-neutralizing antibody inhibited S1P-enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P-enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.