Preparation of nanoscale Bacillus thuringiensis chitinases using silica nanoparticles for nematicide delivery
Preparation of nanoscale Bacillus thuringiensis chitinases using silica nanoparticles for nematicide delivery
复制标题
使用二氧化硅纳米颗粒制备纳米级苏云金芽孢杆菌几丁质酶用于杀线虫剂递送
DOI：
10.1016/j.ijbiomac.2015.10.030
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发表时间：
2016-01-01
影响因子：
8.2
通讯作者：
Li, Lin
中科院分区：
文献类型：
--
作者：
Qin, Xu;Xiang, Xuemei;Li, Lin
基于SiO2 NYSi 4 O纳米颗粒制备了一系列氨基、羧基和羧基表面接枝的二氧化硅纳米颗粒（SNPs），以开发一种高效、生物相容性和成本效益高的生物农药释放系统。以苏云金杆菌几丁质酶（Chi 9602）为载体，通过静电吸附和共价结合的方法，将其固定在SNP表面，制备纳米级几丁质酶（SNPCs）。通过傅里叶变换红外光谱、扫描电子显微镜和zeta电位分析对样品进行了表征。通过免疫荧光法观察秀丽隐杆线虫N2中SNPs的递送能力。结果表明，在所有SNPC中，表面静电吸附Chi 9602的氨基接枝SiO2纳米粒子（SNPC 2）具有较高的酶固定化率（80.2%）和最高的酶残留活性（94.1%）。SNPC 2还表现出比Chi 9602更宽的pH耐受性和相对更高的热稳定性和抗紫外线辐射能力。生物测定进一步表明SNPC 2协同增强了B的杀线虫作用。苏云金杆菌YBT-020制剂对C.对线虫的LC_（50）为8.35 mg/mL，LT_（50）为12.04 h。免疫荧光分析表明SNPC 2具有相当大的运载能力，可将大蛋白质运载到C.优美的因此，SNP 2可以作为一种有效的纳米载体，用于递送大分子蛋白质生物农药或药物，表明潜在的农业或生物技术应用。(C)2015爱思唯尔B. V.保留所有权利。
A series of amino, carboxylic, and aldehydic surface-grafted silica nanoparticles (SNPs) was prepared based on SiO2 NYSi4O nanoparticles to develop an efficient, biocompatible, and cost-effective biopesticide delivery system. Bacillus thuringiensis chitinase (Chi9602) was immobilized onto SNP surface to prepare nanoscale chitinases (SNPCs) through electrostatic adsorption and covalent binding. The specimens were characterized by Fourier transform infrared, scanning electron microscopy, and zeta-potential analyses. The delivery capacity of the SNPs in Caenorhabditis elegans N2 was observed by immunofluorescence. Results demonstrated that amino-grafted SiO2 nanoparticles with Chi9602 electrostatically adsorbed onto their surface (SNPC2) exhibited a relatively high enzyme immobilization rate (80.2%) and the highest (94.1%) residual enzyme activity among all SNPCs. SNPC2 also showed wider pH tolerance and relatively higher thermostability and ultraviolet radiation resistance capacity than Chi9602. Bioassays further showed that SNPC2 synergistically enhanced the nematicidal effect of B. thuringiensis YBT-020 preparation against C. elegans, with a reduced LC50 of 8.35 mg/mL and a shortened LT50 of 12.04h. Immunofluorescence assays showed that SNPC2 had considerable delivery capacity to carry a large protein into C. elegans. Therefore, SNP2 can serve as an efficient nanocarrier for the delivery of macromolecular proteic biopesticides or drugs, indicating potential agricultural or biotechnological applications. (C) 2015 Elsevier B.V. All rights reserved.