Scaffold and growth factor selection in temporomandibular joint disc engineering
Scaffold and growth factor selection in temporomandibular joint disc engineering
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DOI：
10.1177/154405910808700205
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发表时间：
2008-02-01
影响因子：
7.6
通讯作者：
Athanasiou, K. A.
中科院分区：
文献类型：
--
作者：
Allen, K. D.;Athanasiou, K. A.
颞下颌关节盘组织工程研究通常不能在构建收缩之前产生有意义的基质。我们假设，与聚乙醇酸(PGA)非织造网相比，聚L-乳酸(PLLA)非织造网将限制收缩，允许进行全面的力学评估，并保持生存能力。此外，我们提出，生长因子刺激虽然限制收缩，但与以前的报告相比，将增加结构特性。4周后，两种材料的细胞增殖和基质沉积相似，但PGA结构明显收缩。此外，只有PLLA结构可以进行拉伸和压缩测试。用胰岛素样生长因子-1(10 ng/m L)、转化生长因子-β1(5 ng/m L)或转化生长因子-β3(5 ng/m L)处理PLLA构建物。转化生长因子-β1在6周时产生了最多的细胞、胶原和糖胺聚糖；这些结构也显示出改善的力学性能。对这些数据的分析表明，PLLA和转化生长因子-β1具有显著的颞下颌关节去工程化潜力。
Temporomandibular joint disc tissue-engineering studies commonly fail to produce significant matrix before construct contraction. We hypothesized that poly-L-lactic acid (PLLA) non-woven meshes would limit contraction, allow for comprehensive mechanical evaluation, and maintain viability relative to polyglycolic acid (PGA) non-woven mesh controls. Additionally, we proposed that growth factor stimulation, while limiting contraction, would increase construct properties relative to previous reports. After 4 wks, cell proliferation and matrix deposition were similar between the two meshes, but PGA constructs had contracted significantly. Furthermore, only PLLA constructs could be tested in tension and compression. Additional PLLA constructs were formed, then treated with insulin-like growth factor-1 (10 ng/mL), transforming growth factor-beta 1 (5 ng/mL), or transforming growth factor-beta 3 (5 ng/mL). Transforming growth factor-beta 1 yielded the most cells, collagen, and glycosaminoglycans at 6 wks; these constructs also demonstrated improved mechanics. Analysis of these data demonstrated significant temporomandibular joint discengineering potential for PLLA and transforming growth factor-beta 1.