Analysis of petal anthocyanins to investigate coloration mechanism in herbaceous peony cultivars
Analysis of petal anthocyanins to investigate coloration mechanism in herbaceous peony cultivars
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花瓣花色苷分析研究芍药品种的着色机制
DOI：
10.1016/j.scienta.2008.03.016
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发表时间：
2008-06-26
影响因子：
4.3
通讯作者：
Liu, Zheng-An
中科院分区：
文献类型：
--
作者：
Jia, Ni;Shu, Qing-Yan;Liu, Zheng-An
对41个芍药品种的花瓣颜色和花色苷成分进行了分析。采用高效液相色谱-电喷雾电离-质谱(HPLC-ESI-MSn) -光电二极管阵列检测（DAD）对花青素进行鉴定。芍药-3,5-二- o -葡萄糖苷（Pn3G5G）、芍药-3,5-二- o -葡萄糖苷（Pg3G5G）、花青素-3,5-二- o -葡萄糖苷（Cy3G5G）、芍药-3- o -葡萄糖苷（Pn3G）和花青素-3- o -葡萄糖苷（Cy3G）是芍药品种中主要的5种花青素。深紫色和红紫色品种含有4-5种花青素，而粉红色品种只含有Cy3G5G和Pn3G5G，其含量远低于紫色品种。根据与花瓣颜色相关的三种花青素的化学结构，将花分为三种表型：1。“Pn, Cy，和Pg”（全是紫色的花，包括两朵粉红色的花）：2。“Pn, Cy”（粉色花朵）：3“Pn”（浅粉色和白色花朵）。粉红色和紫色花品种的显色机制有很大差异。亮度（L*）与色度（C*）、色度组分a*与总花青素（TA）值、a*与共色素沉着指数（CI）呈相反趋势，而L*与TA呈相同趋势。Pn3G5G和Pg3G5G含量高可能是芍药品种呈紫色的原因。(C) 2008 Elsevier B.V.版权所有
Petal coloration and anthocyanin compositions of 41 herbaceous peony cultivars were analyzed. Anthocyanins were identified by high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MSn) coupled with photodiode array detection (DAD). Peonidin-3,5-di-O-glucoside (Pn3G5G), pelargonidin-3,5-di-O-glucoside (Pg3G5G), cyanidin-3,5-di-O-glucoside (Cy3G5G), peonidin-3-O-glucoside (Pn3G), and cyanidin-3-O-glucoside (Cy3G) were the five major anthocyanins in herbaceous peony cultivars. Deep purple or reddish purple cultivars contained 4-5 anthocyanins, whereas pink cultivars only contained Cy3G5G and Pn3G5G, and their contents were much lower than those of purple cultivars. According to the chemical structures of three anthocyanidins in association with petal coloration, flowers were classified into three phenotypes: 1. "Pn, Cy, and Pg" (all purple flowers including two pink flowers): 2. "Pn, Cy" (pink flowers): 3 "Pn" (light pink and white flowers). The coloration mechanisms of cultivars with the pink and purple flowers were quite different. Correlations between lightness (L*) and chroma (C*), chromatic component a* and total anthocyanins (TA) value, a* and co-pigmentation index (CI) showed opposite tendencies, whereas L* and TA showed the same tendency in each group. High contents of Pn3G5G and Pg3G5G may responsible for the purple coloration of herbaceous peony cultivars. (C) 2008 Elsevier B.V. All rights reserved.