Retention mechanism and enrichment of glycopeptides on titanium dioxide
Retention mechanism and enrichment of glycopeptides on titanium dioxide
复制标题
二氧化钛上糖肽的保留机制及富集
DOI：
10.1039/c3ay41294f
复制
发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Liang, Xinmiao
中科院分区：
文献类型：
--
作者：
Sheng, Qianying;Li, Xiuling;Liang, Xinmiao
提出了一种用二氧化钛（TiO 2）对糖肽进行简便和选择性富集的统一方法。通过考察乙腈含量、NH3中心点H2O浓度和甲酸浓度对糖肽保留的影响，探讨了糖肽与TiO 2之间的保留机理。糖肽和TiO 2之间的亲水相互作用和配体交换保留机制被发现参与。在此基础上，建立了一种均匀的二氧化钛选择性富集糖肽的方法。所开发的方法表现出高选择性的N-连接的糖肽与短到长的肽序列。这种高效、统一的方法将为蛋白质组学中的翻译后修饰研究提供一个有价值的工具。
A uniform method for the facile and selective enrichment of glycopeptides with titanium dioxide (TiO2) is presented. Retention mechanisms between glycopeptides and TiO2 were explored by checking the influence of acetonitrile content, NH3 center dot H2O concentration and formic acid concentration on the retention of glycopeptides. Both hydrophilic interaction and ligand-exchange retention mechanisms between glycopeptides and TiO2 were found to be involved. Based on this, a uniform method was established for selective enrichment of glycopeptides with TiO2. The developed method exhibited high selectivity for N-linked glycopeptides with short to long peptide sequences. This efficient and uniform method will be a valuable tool for post-translational modification in proteomics.