Vesicle fusion proteins in rat inner medullary collecting duct and amphibian bladder.
Vesicle fusion proteins in rat inner medullary collecting duct and amphibian bladder.
复制标题
大鼠内髓集合管和两栖动物膀胱中的囊泡融合蛋白。
DOI：
10.1152/ajpcell.1995.268.3.c792
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hays,RM
中科院分区：
文献类型：
--
作者：
Franki,N;Macaluso,F;Gao,Y;Hays,RM
响应于抗利尿激素（ADH）将水通道递送至顶膜需要将含有通道的囊泡靶向至膜中的特定位点，随后融合和胞吐。现在认为一系列复杂的蛋白质介导真核细胞中的靶向和融合。它们包括N-乙基马来酰亚胺敏感性融合蛋白（NSF）、可溶性NSF附着蛋白（SNAP）和纤维泡蛋白（cellubrevin），一种存在于神经末梢的囊泡相关蛋白。我们问这些蛋白质是否在上皮细胞的大鼠内髓集合管（IMCD）和两栖类膀胱。两种组织上的免疫印迹显示NSF和α-SNAP的存在。Cellubrevin存在于IMCD的免疫印迹中，但不是膀胱。免疫金电子显微镜显示NSF，α-SNAP，和cellubrevin在大鼠IMCD细胞，与囊泡标记。在膀胱中，NSF可见于囊泡和聚集体上。我们的结论是，组件的囊泡靶向和融合系统存在于肾脏和两栖类膀胱，并可能介导各种各样的融合事件，包括ADH发起的。
The delivery of water channels to the apical membrane in response to antidiuretic hormone (ADH) requires the targeting of channel-containing vesicles to specific sites in the membrane, followed by fusion and exocytosis. A complex array of proteins is now believed to mediate targeting and fusion in eukaryotic cells. They include N-ethylmaleimide-sensitive fusion protein (NSF), soluble NSF attachment proteins (SNAP), and cellubrevin, a vesicle-associated protein present in the nerve terminal. We asked whether these proteins are in epithelial cells of rat inner medullary collecting duct (IMCD) and amphibian bladder. Immunoblots on both tissues showed the presence of NSF and alpha-SNAP. Cellubrevin was present in immunoblots of the IMCD, but not the bladder. Immunogold electron microscopy showed NSF, alpha-SNAP, and cellubrevin in rat IMCD cells, with vesicular labeling. In the bladder, NSF was seen on vesicles and aggrephores. We conclude that components of the vesicle-targeting and fusion systems are present in kidney and amphibian bladder and may mediate a wide variety of fusion events, including those initiated by ADH.