In Vitro Antibacterial Activity of Phx-3 against Helicobacter pylori
In Vitro Antibacterial Activity of Phx-3 against Helicobacter pylori
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DOI：
10.1248/bpb.33.188
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发表时间：
2010-02-01
影响因子：
2
通讯作者：
Kamiya, Shigeru
中科院分区：
文献类型：
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作者：
Hanawa, Tomoko;Osaki, Takako;Kamiya, Shigeru
Phx-3是一种吩恶嗪衍生物，对分枝杆菌和肺炎衣原体有抑制作用，但对大肠杆菌、鼠伤寒沙门氏菌、铜绿假单胞菌、金黄色葡萄球菌、单核细胞增生李斯特菌无抑制作用。尚未评估Phx-3对幽门螺杆菌菌株的杀菌活性。然后，我们测定了Phx-3对幽门螺杆菌的最低抑菌浓度，并评估了Phx-3对H.幽门。本研究表明，H. Phx-3可有效杀死克拉霉素耐药株和鼠螺杆菌等幽门螺杆菌菌株。此外，严重的形态学变化，如膜起泡和空洞的形成，在H。pylori检测。此外，还观察到热休克蛋白60的诱导。综上所述，Phx-3对幽门螺杆菌具有抗菌活性。
Phx-3, one of the phenoxazine derivatives, is reported to have inhibitory effect on Mycobacterium species and Chlamydia pneumoniae but not Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes. The bactericidal activities of Phx-3 against Helicobacter pylori strains have not been assessed. Then, we measured minimum inhibitory concentration of Phx-3 for Helicobacter strains and assessed the morphological and biochemical effects of Phx-3 on H. pylori. In present study, it has shown that H. pylori strains including clarithromycin resistant strain and Helicobacter musterae were killed effectively by the treatment with Phx-3. Furthermore, severe morphological changes such as membrane blebbing and formation of hollows in H. pylori were detected. In addition, induction of heat shock protein 60 was observed. Taken together, Phx-3 has antibacterial activity against Helicobacter pylori.