Repair of Avascular Meniscus Tears with Electrospun Collagen Scaffolds Seeded with Human Cells.
Repair of Avascular Meniscus Tears with Electrospun Collagen Scaffolds Seeded with Human Cells.
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DOI：
10.1089/ten.tea.2015.0284
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发表时间：
2016-03
影响因子：
--
通讯作者：
D'Lima DD
中科院分区：
文献类型：
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作者：
Baek J;Sovani S;Glembotski NE;Du J;Jin S;Grogan SP;D'Lima DD
The self-healing capacity of an injured meniscus is limited to the vascularized regions and is especially challenging in the inner avascular regions.因此，我们研究了使用人类半月板细胞接种的电纺（ES）I型胶原蛋白支架来生产半月板组织，并探讨这些细胞接种的支架是否可以植入以修复半月板无血管组织外植体中产生的缺陷。 Human meniscal cells (derived from vascular and avascular meniscal tissue) were seeded on ES scaffolds and cultured. Constructs were evaluated for cell viability, gene expression, and mechanical properties.为了确定半月板缺损修复的潜力，在植入前将人半月板无血管细胞接种在对齐的 ES 胶原支架上并培养 4 周。在牛半月板的无血管区制造类似“纵向撕裂”的手术缺陷，并植入细胞接种的胶原支架并培养 3 周。 Tissue regeneration and integration were evaluated by histology, immunohistochemistry, mechanical testing, and magentic resonance imaging.与无细胞胶原支架或未经治疗的缺陷相比，体外植入细胞接种的胶原支架产生的新组织与天然组织的整合明显更好。 Human meniscal cell-seeded ES collagen scaffolds may therefore be useful in facilitating meniscal repair of avascular meniscus tears.
The self-healing capacity of an injured meniscus is limited to the vascularized regions and is especially challenging in the inner avascular regions. As such, we investigated the use of human meniscus cell-seeded electrospun (ES) collagen type I scaffolds to produce meniscal tissue and explored whether these cell-seeded scaffolds can be implanted to repair defects created in meniscal avascular tissue explants. Human meniscal cells (derived from vascular and avascular meniscal tissue) were seeded on ES scaffolds and cultured. Constructs were evaluated for cell viability, gene expression, and mechanical properties. To determine potential for repair of meniscal defects, human meniscus avascular cells were seeded and cultured on aligned ES collagen scaffolds for 4 weeks before implantation. Surgical defects resembling “longitudinal tears” were created in the avascular zone of bovine meniscus and implanted with cell-seeded collagen scaffolds and cultured for 3 weeks. Tissue regeneration and integration were evaluated by histology, immunohistochemistry, mechanical testing, and magentic resonance imaging. Ex vivo implantation with cell-seeded collagen scaffolds resulted in neotissue that was significantly better integrated with the native tissue than acellular collagen scaffolds or untreated defects. Human meniscal cell-seeded ES collagen scaffolds may therefore be useful in facilitating meniscal repair of avascular meniscus tears.