Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition
Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition
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DOI：
10.3390/pathogens8040297
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发表时间：
2019-12-01
期刊：
影响因子：
3.7
通讯作者：
Matthews, Qiana L.
中科院分区：
文献类型：
--
作者：
Jones, Leandra B.;Kumar, Sanjay;Matthews, Qiana L.
细胞外囊泡（EV）内的分子组分的包装允许它们参与细胞间通讯和生物分子的转移，然而EV在细菌感染期间的作用知之甚少。本研究的目的是检查铜绿假单胞菌（P. aeruginosa）感染对来自小鼠小胶质细胞系BV-2的EV的生物发生和组成的影响。BV-2细胞在无外泌体的培养基中培养，并用0、1.3 x 10（4）或2.6 x 10（4）菌落形成单位/mL铜绿假单胞菌感染72 h。结果表明，与对照组相比，铜绿假单胞菌感染后BV-2细胞活力显著降低，BV-2衍生的EV浓度显著降低。与对照组相比，铜绿假单胞菌感染显著降低了BV-2衍生感染EV中趋化因子配体4信使RNA（p
The packaging of molecular constituents inside extracellular vesicles (EVs) allows them to participate in intercellular communication and the transfer of biological molecules, however the role of EVs during bacterial infection is poorly understood. The goal of this study was to examine the effects of Pseudomonas aeruginosa (P. aeruginosa) infection on the biogenesis and composition of EVs derived from the mouse microglia cell line, BV-2. BV-2 cells were cultured in exosome-free media and infected with 0, 1.3 x 10(4), or 2.6 x 10(4) colony forming units per milliliter P. aeruginosa for 72 h. The results indicated that compared with the control group, BV-2 cell viability significantly decreased after P. aeruginosa infection and BV-2-derived EVs concentration decreased significantly in the P. aeruginosa-infected group. P. aeruginosa infection significantly decreased chemokine ligand 4 messenger RNA in BV-2-derived infected EVs, compared with the control group (p