Memory B cell subpopulations in the aged
Memory B cell subpopulations in the aged
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DOI：
10.1089/rej.2006.9.149
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发表时间：
2006-03-01
影响因子：
2.6
通讯作者：
Caruso, C
中科院分区：
文献类型：
--
作者：
Colonna-Romano, G;Aquino, A;Caruso, C
免疫衰老的文献主要集中在T细胞损伤。为探讨B细胞免疫衰老的机制，作者测定了24例青年人和21例老年人血清IgD水平，并分析了其与CD 19 + CD 27+记忆细胞数量的关系。采用免疫扩散法测定血清IgD水平，流式细胞仪检测淋巴细胞亚群CD 19 + CD 27+。老年人血清IgD水平显著降低（p < 0.0001），而CD 19 + CD 27+淋巴细胞百分比显著升高（p = 0.01）。最后，IgD和CD 19 + CD 27+的血清浓度之间存在显著的负相关性（p = 0.01）。目前的结果表明，IgD的水平是负相关的B记忆细胞的量的年龄。这表明，B库可用于响应新的抗原的挑战是减少老年人。事实上，许多记忆IgDB细胞填充免疫学空间，并且初始IgD+ B细胞的数量显著减少。因此，这些初步结果表明，幼稚IgD+ CD 27-B细胞的减少和记忆IgD-CD 27 + B细胞的伴随增加可能代表B免疫衰老的标志，可能提供与人类寿命相关的生物标志物，并且可能用于评估抗衰老治疗。
The literature on immunosenescence has focused mainly on T cell impairment. With the aim of gaining insight into B cell immunosenescence, the authors investigated the serum IgD levels in 24 young and 21 old people and analyzed their relationship with the number of CD19+CD27+ memory cells. Serum IgD were quantified by the use of radial immunodiffusion and the lymphocyte population CD19+CD27+ was identified by a FACScan flow cytometer. Serum IgD levels were significantly lower (p < 0.0001) in old subjects, and the percentage of CD19+CD27+ lymphocytes were significantly increased (p = 0.01) in old subjects. Finally, a significant negative correlation was found (p = 0.01) between serum concentrations of IgD and CD19+CD27+. The present results show that the levels of IgD are negatively age-related to the amount of B memory cells. This suggests that the B repertoire available to respond to new antigenic challenges is decreased in the elderly. In fact, many memory IgDB cells fill immunologic space, and the number of naive IgD+ B cells is dramatically decreased. Therefore, these preliminary results suggest that a decrease of naive IgD+CD27-B cells and a concomitant increase of memory IgD-CD27+ B cells could represent hallmarks of B immunosenescence, might provide biomarkers related to the lifespan of humans, and could be useful for the evaluation of antiaging treatments.