Wnt signalling required for expansion of neural crest and CNS progenitors
Wnt signalling required for expansion of neural crest and CNS progenitors
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DOI：
10.1038/40146
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发表时间：
1997-10-30
期刊：
影响因子：
64.8
通讯作者：
Takada, S
中科院分区：
文献类型：
--
作者：
Ikeya, M;Lee, SMK;Takada, S
细胞之间的相互作用有助于阐述脊椎动物中枢神经系统（CNS）内的模式（1）。编码富含半胱氨酸的分泌信号的Wnt家族成员的基因Wnt-1和Wnt-3a在发育中的神经管的背中线共表达，与背侧图案形成一致（2，3）。每种信号对胚胎发育都是必不可少的，Wnt-1对中脑模式化（4，5）和Wnt-3a对近轴中胚层的形成（6），但在每种突变体中缺乏背神经管表型表明Wnt信号可能是多余的。在这里，我们证明了在Wnt-1和Wnt-3a都不存在的情况下，神经嵴衍生物明显缺乏，其起源于背侧神经管（7），并且神经管本身内的背外侧神经前体明显减少。这些表型似乎并不导致中断的机制负责建立正常的背腹极性。相反，我们的研究结果是一致的模型，其中本地Wnt信号调节背神经前体的扩张。鉴于不同Wnt基因在哺乳动物神经管的离散区域中广泛表达（3），这可能代表了发育中CNS中Wnt信号传导作用的一般模型。
Interactions between cells help to elaborate pattern within the vertebrate central nervous system (CNS)(1). The genes Wnt-1 and Wnt-3a, which encode members of the Wnt family of cysteine-rich secreted signals, are coexpressed at the dorsal midline of the developing neural tube, coincident with dorsal patterning(2,3). Each signal is essential for embryonic development, Wnt-1 for midbrain patterning(4,5) and Wnt-3a for formation of the paraxial mesoderm(6), but the absence of a dorsal neural-tube phenotype in each mutant suggests that Wnt signalling may be redundant. Here we demonstrate that in the absence of both Wnt-1 and Wnt-3a there is a marked deficiency in neural crest derivatives, which originate from the dorsal neural tube(7), and a pronounced reduction in dorsolateral neural precursors within the neural tube itself. These phenotypes do not seem to result from a disruption in the mechanisms responsible for establishing normal dorsoventral polarity. Rather, our results are consistent with a model in which local Wnt signalling regulates the expansion of dorsal neural precursors. Given the widespread expression of different Wnt genes in discrete areas of the mammalian neural tube(3), this may represent a general model for the action of Wnt signalling in the developing CNS.