The human cumulus-oocyte complex gene-expression profile
The human cumulus-oocyte complex gene-expression profile
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DOI：
10.1093/humrep/del065
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发表时间：
2006-07-01
影响因子：
6.1
通讯作者：
Hamamah, Samir
中科院分区：
文献类型：
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作者：
Assou, Said;Anahory, Tal;Hamamah, Samir
背景：对人类卵母细胞成熟调控机制的认识还很不成熟。我们已经确定了未成熟和成熟卵母细胞和卵丘细胞之间差异表达的转录本。方法：使用寡核苷酸微阵列，在接受IVF的患者的未成熟和成熟卵母细胞或卵丘细胞中研究全基因组基因表达。结果：除DAZL、BMP 15、GDF 9等已知基因外，卵母细胞共上调1514个基因。我们发现PTTG 3和AURKC分别是在卵母细胞减数分裂过程中优先表达的securin和Aurora激酶。引人注目的是，卵母细胞过度表达以前未报道的生长因子，如TNFSF 13/APRIL，FGF 9，FGF 14和IL 4以及转录因子，包括OTX 2，SOX 15和SOX 30。相反，卵丘细胞，除了已知的基因，如LHGR或BMPR 2，过表达细胞间信号传导基因，包括TNFSF 11/RANKL，许多补体成分，信号蛋白（SEMA 3A，SEMA 6A和SEMA 6D）和CD基因，如CD 200。我们还鉴定了52个在卵母细胞成熟过程中逐渐增加的基因，包括CDC 25 A和SOCS 7。结论：在卵母细胞成熟过程中上调和下调的基因的鉴定大大提高了我们对卵母细胞生物学的理解，并将提供新的标志物，信号可行的和有能力的卵母细胞。此外，发现在卵丘细胞中表达的基因是颗粒细胞肿瘤的潜在标志物。
BACKGROUND: The understanding of the mechanisms regulating human oocyte maturation is still rudimentary. We have identified transcripts differentially expressed between immature and mature oocytes and cumulus cells. METHODS: Using oligonucleotide microarrays, genome-wide gene expression was studied in pooled immature and mature oocytes or cumulus cells from patients who underwent IVF. RESULTS: In addition to known genes, such as DAZL, BMP15 or GDF9, oocytes up-regulated 1514 genes. We show that PTTG3 and AURKC are respectively the securin and the Aurora kinase preferentially expressed during oocyte meiosis. Strikingly, oocytes overexpressed previously unreported growth factors such as TNFSF13/APRIL, FGF9, FGF14 and IL4 and transcription factors including OTX2, SOX15 and SOX30. Conversely, cumulus cells, in addition to known genes such as LHCGR or BMPR2, overexpressed cell-to-cell signalling genes including TNFSF11/RANKL, numerous complement components, semaphorins (SEMA3A, SEMA6A and SEMA6D) and CD genes such as CD200. We also identified 52 genes progressively increasing during oocyte maturation, including CDC25A and SOCS7. CONCLUSION: The identification of genes that were up- and down-regulated during oocyte maturation greatly improves our understanding of oocyte biology and will provide new markers that signal viable and competent oocytes. Furthermore, genes found expressed in cumulus cells are potential markers of granulosa cell tumours.